Psb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii

Natsuko Inoue-Kashino, Yasuhiro Kashino, Yuichiro Takahashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The psb30 (ycf12) gene is conserved in a wide variety of oxygenic-photosynthetic organisms except angiosperms and some marine cyanobacteria. Psb30 protein is found in cyanobacterial photosystem II (PSII) core complexes and is dispensable for PSII structure and function. The most recent three-dimensional structure of cyanobacterial PSII core complex has revealed that Psb30 is located in proximity of PsbJ, PsbK, and PsbZ. However Psb30 has not yet been detected in PSII complexes from eukaryotic photosynthetic organisms. Here we found the expression of the chloroplast psb30 gene in the green alga Chlamydomonas reinhardtii by immunoblotting and Psb30 is exclusively co-purifies with PSII core complex and is significantly reduced in PSII-deficient mutants. Partial disintegration of PSII core complex and subsequent fractionation of the resulting subcomplexes revealed that Psb30 is exclusively associated with PSII reaction center. We have generated chloroplast transformants in which the psb30 gene is disrupted and the resulting ΔPsb30 cells showed decreased oxygen evolution activity by 15%, grew photosynthetically under moderate light, and displayed increased sensitivity to high light relative to wild type. We conclude that Psb30 is a PSII reaction center subunit and is required for optimal PSII function under high light environments.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume104
Issue number1-2
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Chlamydomonas reinhardtii
Photosystem II Protein Complex
genes
chloroplasts
Light
Growth
organisms
angiosperms
disintegration
algae
fractionation
proximity
proteins
Chloroplast Genes
sensitivity
Photosynthetic Reaction Center Complex Proteins
oxygen
Angiosperms
cells
Chlorophyta

Keywords

  • Chlamydomonas reinhardtii
  • Low-molecular-weight polypeptide
  • Photoinhibition
  • Photosystem II
  • psb30
  • ycf12

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Psb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii. / Inoue-Kashino, Natsuko; Kashino, Yasuhiro; Takahashi, Yuichiro.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 104, No. 1-2, 07.2011, p. 220-228.

Research output: Contribution to journalArticle

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