Protocols for screening peptide motifs binding to ccn family proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Function of CCN family proteins is determined by the interactions with multiple cofactors that are present in microenvironment. Therefore, finding out these cofactors is critically important in understanding the molecular function of the CCN family members. For this objective, bacteriophage random peptide display library is a quite feasible tool. In this library, each filamentous bacteriophage is designed to display an oligopeptide of random 12–16 amino acid residues on its surface. Bacteriophage clones that possess the peptides that bind to a CCN family protein are selected through several cycles of a process designated biopanning or affinity selection. By determining the nucleotide sequence of the DNA that encodes the displayed peptide, oligopeptides that specifically bind to the CCN family member can be specified. Obtained peptide sequences can be utilized for designing peptide aptamers for the CCN family protein, or as a key sequence to find out new CCN family cofactor candidates in silico.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-167
Number of pages13
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

CCN Intercellular Signaling Proteins
Oligopeptides
Bacteriophages
Peptides
Peptide Aptamers
Inovirus
Peptide Library
Proteins
Computer Simulation
Libraries
Clone Cells
Amino Acids
DNA

Keywords

  • Aptamer
  • Bacteriophage
  • CCN family
  • M13
  • Phage display

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kubota, S., Kawaki, H., & Takigawa, M. (2017). Protocols for screening peptide motifs binding to ccn family proteins. In Methods in Molecular Biology (Vol. 1489, pp. 155-167). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_16

Protocols for screening peptide motifs binding to ccn family proteins. / Kubota, Satoshi; Kawaki, Harumi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 155-167 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kubota, S, Kawaki, H & Takigawa, M 2017, Protocols for screening peptide motifs binding to ccn family proteins. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 155-167. https://doi.org/10.1007/978-1-4939-6430-7_16
Kubota S, Kawaki H, Takigawa M. Protocols for screening peptide motifs binding to ccn family proteins. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 155-167. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_16
Kubota, Satoshi ; Kawaki, Harumi ; Takigawa, Masaharu. / Protocols for screening peptide motifs binding to ccn family proteins. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 155-167 (Methods in Molecular Biology).
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