Proteomics profiling of arginine methylation defines PRMT5 substrate specificity

Daniele Musiani, Jabez Bok, Enrico Massignani, Liling Wu, Tommaso Tabaglio, Marica Rosaria Ippolito, Alessandro Cuomo, Umut Ozbek, Habiba Zorgati, Umesh Ghoshdastider, Robert C. Robinson, Ernesto Guccione, Tiziana Bonaldi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Protein arginine methyltransferases (PRMTs) catalyze arginine methylation on both chromatin-bound and cytoplasmic proteins. Accumulating evidence supports the involvement of PRMT5, the major type II PRMT, in cell survival and differentiation pathways that are important during development and in tumorigenesis. PRMT5 is an attractive drug target in various cancers, and inhibitors are currently in oncological clinical trials. Nonetheless, given the complex biology of PRMT5 and its multiple nonhistone substrates, it is paramount to fully characterize these dynamic changes in methylation and to link them to the observed anticancer effects to fully understand the functions of PRMT5 and the consequences of its inhibition. Here, we used a newly established pipeline coupling stable isotope labeling with amino acids in cell culture (SILAC) with immunoenriched methyl peptides to globally profile arginine monomethylation and symmetric dimethylation after PRMT5 inhibition by a selective inhibitor. We adopted heavy methyl SILAC as an orthogonal validation method to reduce the false discovery rate. Through in vitro methylation assays, we validated a set of PRMT5 targets identified by mass spectrometry and provided previously unknown mechanistic insights into the preference of the enzyme to methylate arginine sandwiched between two neighboring glycines (a Gly-Arg-Gly, or “GRG,” sequence). Our analysis led to the identification of previously unknown PRMT5 substrates, thus both providing insight into the global effects of PRMT5 and its inhibition in live cells, beyond chromatin, and refining our knowledge of its substrate specificity.

Original languageEnglish
Article numbereaat8388
JournalScience Signaling
Volume12
Issue number575
DOIs
Publication statusPublished - Apr 2 2019
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Musiani, D., Bok, J., Massignani, E., Wu, L., Tabaglio, T., Ippolito, M. R., Cuomo, A., Ozbek, U., Zorgati, H., Ghoshdastider, U., Robinson, R. C., Guccione, E., & Bonaldi, T. (2019). Proteomics profiling of arginine methylation defines PRMT5 substrate specificity. Science Signaling, 12(575), [eaat8388]. https://doi.org/10.1126/scisignal.aat8388