Proteomics-based analysis of invasion-related proteins in malignant gliomas

Tomoko Maruo, Tomotsugu Ichikawa, Hirotaka Kanzaki, Satoshi Inoue, Kazuhiko Kurozumi, Manabu Onishi, Koichi Yoshida, Hirokazu Kambara, Mamoru Ouchida, Kenji Shimizu, Seiji Tamaru, E. Antonio Chiocca, Isao Date

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

One of the insidious biological features of gliomas is their potential to extensively invade normal brain tissue, yet molecular mechanisms that dictate this locally invasive behavior remain poorly understood. To investigate the molecular basis of invasion by malignant gliomas, proteomic analysis was performed using a pair of canine glioma subclones - J3T-1 and J3T-2 - that show different invasion phenotypes in rat brains but have similar genetic backgrounds. Two-dimensional protein electrophoresis of whole-cell lysates of J3T-1 (angiogenesis-dependent invasion phenotype) and J3T-2 (angiogenesis-independent invasion phenotype) was performed. Twenty-two distinct spots were recognized when significant alteration was defined as more than 1.5-fold change in spot intensity between J3T-1 and J3T-2. Four proteins that demonstrated increased expression in J3T-1, and 14 proteins that demonstrated increased expression in J3T-2 were identified using liquid chromatography-mass spectrometry analysis. One of the proteins identified was annexin A2, which was expressed at higher levels in J3T-1 than in J3T-2. The higher expression of annexin A2 in J3T-1 was corroborated by quantitative RT-PCR of the cultured cells and immunohistochemical staining of the rat brain tumors. Moreover, immunohistochemical analysis of human glioblastoma specimens showed that annexin A2 was expressed at high levels in the tumor cells that formed clusters around dilated vessels. These results reveal differences in the proteomic profiles between these two cell lines that might correlate with their different invasion profiles. Thus, annexin A2 may be related to angiogenesis-dependent invasion.

Original languageEnglish
Pages (from-to)264-275
Number of pages12
JournalNeuropathology
Volume33
Issue number3
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Annexin A2
Glioma
Proteomics
Phenotype
Proteins
Brain
Glioblastoma
Brain Neoplasms
Liquid Chromatography
Electrophoresis
Canidae
Cultured Cells
Mass Spectrometry
Staining and Labeling
Cell Line
Polymerase Chain Reaction
Neoplasms

Keywords

  • Angiogenesis
  • Annexin A2
  • Glioma
  • Invasion
  • Proteomics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Proteomics-based analysis of invasion-related proteins in malignant gliomas. / Maruo, Tomoko; Ichikawa, Tomotsugu; Kanzaki, Hirotaka; Inoue, Satoshi; Kurozumi, Kazuhiko; Onishi, Manabu; Yoshida, Koichi; Kambara, Hirokazu; Ouchida, Mamoru; Shimizu, Kenji; Tamaru, Seiji; Chiocca, E. Antonio; Date, Isao.

In: Neuropathology, Vol. 33, No. 3, 06.2013, p. 264-275.

Research output: Contribution to journalArticle

Maruo, T, Ichikawa, T, Kanzaki, H, Inoue, S, Kurozumi, K, Onishi, M, Yoshida, K, Kambara, H, Ouchida, M, Shimizu, K, Tamaru, S, Chiocca, EA & Date, I 2013, 'Proteomics-based analysis of invasion-related proteins in malignant gliomas', Neuropathology, vol. 33, no. 3, pp. 264-275. https://doi.org/10.1111/j.1440-1789.2012.01361.x
Maruo, Tomoko ; Ichikawa, Tomotsugu ; Kanzaki, Hirotaka ; Inoue, Satoshi ; Kurozumi, Kazuhiko ; Onishi, Manabu ; Yoshida, Koichi ; Kambara, Hirokazu ; Ouchida, Mamoru ; Shimizu, Kenji ; Tamaru, Seiji ; Chiocca, E. Antonio ; Date, Isao. / Proteomics-based analysis of invasion-related proteins in malignant gliomas. In: Neuropathology. 2013 ; Vol. 33, No. 3. pp. 264-275.
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