Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain

Haruko Ota, Takao Hikita, Tomoki Nishioka, Mami Matsumoto, Jun Ito, Naoya Asai, Atsushi Enomoto, Masahide Takahashi, Kozo Kaibuchi, Kazuya Sobue, Kazunobu Sawamoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Neural stem cells continuously generate new neurons in the ventricular-subventricular zone (V-SVZ) of the postnatal and adult mammalian brain. New neurons born in the rodent V-SVZ migrate toward the olfactory bulb (OB), where they differentiate into interneurons. To reveal novel intracellular molecular mechanisms that control postnatal neuronal migration, we performed a global proteomic search for proteins interacting with Girdin, an essential protein for postnatal neuronal migration. Using GST pull-down and LC-MS/MS shotgun analysis, we identified cytoskeletal proteins, cytoskeleton-binding proteins, and signal-transduction proteins as possible participants in neuronal migration. Our results suggest that Girdin and Girdin-interacting proteins control neuronal migration by regulating actin and/or microtubule dynamics.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1-2
Publication statusPublished - Dec 6 2013
Externally publishedYes


  • Girdin
  • Liquid chromatography tandem mass spectrometry (LC-MS/MS)
  • Neuronal migration
  • Postnatal neurogenesis
  • Ventricular-subventricular zone (V-SVZ)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain'. Together they form a unique fingerprint.

Cite this