Proteomic analysis from the mineralized radular teeth of the giant Pacific chiton, Cryptochiton stelleri (Mollusca)

Michiko Nemoto, Qianqian Wang, Dongsheng Li, Songqin Pan, Tadashi Matsunaga, David Kisailus

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The biomineralized radular teeth of chitons are known to consist of iron-based magnetic crystals, associated with the maximum hardness and stiffness of any biomineral. Based on our transmission electron microscopy analysis of partially mineralized teeth, we suggest that the organic matrix within the teeth controls the iron oxide nucleation. Thus, we used Nano-LC-MS to perform a proteomic analysis of the organic matrix in radular teeth of the chiton Cryptochiton stelleri in order to identify the proteins involved in the biomineralization process. Since the genome sequence of C. stelleri is not available, cross-species similarity searching and de novo peptide sequencing were used to screen the proteins. Our results indicate that several proteins were dominant in the mineralized part of the radular teeth, amongst which, myoglobin and a highly acidic peptide were identified as possibly involved in the biomineralization process.

Original languageEnglish
Pages (from-to)2890-2894
Number of pages5
JournalProteomics
Volume12
Issue number18
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Polyplacophora
Mollusca
Proteomics
Biomineralization
Tooth
Peptides
Proteins
Myoglobin
Nucleation
Iron
Genes
Hardness
Stiffness
Transmission electron microscopy
Crystals
Transmission Electron Microscopy
Genome

Keywords

  • Animal proteomics
  • Biomineralization
  • De novo peptide sequencing
  • Radular teeth

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Proteomic analysis from the mineralized radular teeth of the giant Pacific chiton, Cryptochiton stelleri (Mollusca). / Nemoto, Michiko; Wang, Qianqian; Li, Dongsheng; Pan, Songqin; Matsunaga, Tadashi; Kisailus, David.

In: Proteomics, Vol. 12, No. 18, 09.2012, p. 2890-2894.

Research output: Contribution to journalArticle

Nemoto, Michiko ; Wang, Qianqian ; Li, Dongsheng ; Pan, Songqin ; Matsunaga, Tadashi ; Kisailus, David. / Proteomic analysis from the mineralized radular teeth of the giant Pacific chiton, Cryptochiton stelleri (Mollusca). In: Proteomics. 2012 ; Vol. 12, No. 18. pp. 2890-2894.
@article{de24325f4b7a4f5cb596b59e7c87f9c3,
title = "Proteomic analysis from the mineralized radular teeth of the giant Pacific chiton, Cryptochiton stelleri (Mollusca)",
abstract = "The biomineralized radular teeth of chitons are known to consist of iron-based magnetic crystals, associated with the maximum hardness and stiffness of any biomineral. Based on our transmission electron microscopy analysis of partially mineralized teeth, we suggest that the organic matrix within the teeth controls the iron oxide nucleation. Thus, we used Nano-LC-MS to perform a proteomic analysis of the organic matrix in radular teeth of the chiton Cryptochiton stelleri in order to identify the proteins involved in the biomineralization process. Since the genome sequence of C. stelleri is not available, cross-species similarity searching and de novo peptide sequencing were used to screen the proteins. Our results indicate that several proteins were dominant in the mineralized part of the radular teeth, amongst which, myoglobin and a highly acidic peptide were identified as possibly involved in the biomineralization process.",
keywords = "Animal proteomics, Biomineralization, De novo peptide sequencing, Radular teeth",
author = "Michiko Nemoto and Qianqian Wang and Dongsheng Li and Songqin Pan and Tadashi Matsunaga and David Kisailus",
year = "2012",
month = "9",
doi = "10.1002/pmic.201100473",
language = "English",
volume = "12",
pages = "2890--2894",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "18",

}

TY - JOUR

T1 - Proteomic analysis from the mineralized radular teeth of the giant Pacific chiton, Cryptochiton stelleri (Mollusca)

AU - Nemoto, Michiko

AU - Wang, Qianqian

AU - Li, Dongsheng

AU - Pan, Songqin

AU - Matsunaga, Tadashi

AU - Kisailus, David

PY - 2012/9

Y1 - 2012/9

N2 - The biomineralized radular teeth of chitons are known to consist of iron-based magnetic crystals, associated with the maximum hardness and stiffness of any biomineral. Based on our transmission electron microscopy analysis of partially mineralized teeth, we suggest that the organic matrix within the teeth controls the iron oxide nucleation. Thus, we used Nano-LC-MS to perform a proteomic analysis of the organic matrix in radular teeth of the chiton Cryptochiton stelleri in order to identify the proteins involved in the biomineralization process. Since the genome sequence of C. stelleri is not available, cross-species similarity searching and de novo peptide sequencing were used to screen the proteins. Our results indicate that several proteins were dominant in the mineralized part of the radular teeth, amongst which, myoglobin and a highly acidic peptide were identified as possibly involved in the biomineralization process.

AB - The biomineralized radular teeth of chitons are known to consist of iron-based magnetic crystals, associated with the maximum hardness and stiffness of any biomineral. Based on our transmission electron microscopy analysis of partially mineralized teeth, we suggest that the organic matrix within the teeth controls the iron oxide nucleation. Thus, we used Nano-LC-MS to perform a proteomic analysis of the organic matrix in radular teeth of the chiton Cryptochiton stelleri in order to identify the proteins involved in the biomineralization process. Since the genome sequence of C. stelleri is not available, cross-species similarity searching and de novo peptide sequencing were used to screen the proteins. Our results indicate that several proteins were dominant in the mineralized part of the radular teeth, amongst which, myoglobin and a highly acidic peptide were identified as possibly involved in the biomineralization process.

KW - Animal proteomics

KW - Biomineralization

KW - De novo peptide sequencing

KW - Radular teeth

UR - http://www.scopus.com/inward/record.url?scp=84866148380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866148380&partnerID=8YFLogxK

U2 - 10.1002/pmic.201100473

DO - 10.1002/pmic.201100473

M3 - Article

C2 - 22833255

AN - SCOPUS:84866148380

VL - 12

SP - 2890

EP - 2894

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 18

ER -