Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine.

Akira Sato, Eriko Miyazaki, Akito Satake, Akiko Hiramoto, Osamu Hiraoka, Tsuyoshi Miyake, Hye-Sook Kim, Yusuke Wataya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

5-fluoro-2'-deoxyuridine (FUdR) inhibits thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with FUdR. To understand the molecular mechanisms of regulate of two differential cell death necrosis and apoptosis, we identify cell death regulator by using proteome and transcriptome analysis. When compared with the proteome of F28-7 and F28-7-A strain after treated with FUdR, it was found that 5 proteins were up-regulated and 11 proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of necrosis or apoptosis at the proteome and transcriptome levels.

Original languageEnglish
Pages (from-to)433-434
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007

Fingerprint

Gene Expression Profiling
Proteome
Cell Death
Apoptosis
DNA Cleavage
Necrosis
Thymidylate Synthase
Proteins
Transcriptome
Genes
Cell Cycle
Down-Regulation
Breast Neoplasms
5-fluoro-2'-deoxyuridine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sato, A., Miyazaki, E., Satake, A., Hiramoto, A., Hiraoka, O., Miyake, T., ... Wataya, Y. (2007). Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine. Nucleic acids symposium series (2004), (51), 433-434.

Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine. / Sato, Akira; Miyazaki, Eriko; Satake, Akito; Hiramoto, Akiko; Hiraoka, Osamu; Miyake, Tsuyoshi; Kim, Hye-Sook; Wataya, Yusuke.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 433-434.

Research output: Contribution to journalArticle

Sato, A, Miyazaki, E, Satake, A, Hiramoto, A, Hiraoka, O, Miyake, T, Kim, H-S & Wataya, Y 2007, 'Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine.', Nucleic acids symposium series (2004), no. 51, pp. 433-434.
Sato A, Miyazaki E, Satake A, Hiramoto A, Hiraoka O, Miyake T et al. Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine. Nucleic acids symposium series (2004). 2007;(51):433-434.
Sato, Akira ; Miyazaki, Eriko ; Satake, Akito ; Hiramoto, Akiko ; Hiraoka, Osamu ; Miyake, Tsuyoshi ; Kim, Hye-Sook ; Wataya, Yusuke. / Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 433-434.
@article{95513641e8ec48aaa919b7518a2764e0,
title = "Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine.",
abstract = "5-fluoro-2'-deoxyuridine (FUdR) inhibits thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with FUdR. To understand the molecular mechanisms of regulate of two differential cell death necrosis and apoptosis, we identify cell death regulator by using proteome and transcriptome analysis. When compared with the proteome of F28-7 and F28-7-A strain after treated with FUdR, it was found that 5 proteins were up-regulated and 11 proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of necrosis or apoptosis at the proteome and transcriptome levels.",
author = "Akira Sato and Eriko Miyazaki and Akito Satake and Akiko Hiramoto and Osamu Hiraoka and Tsuyoshi Miyake and Hye-Sook Kim and Yusuke Wataya",
year = "2007",
language = "English",
pages = "433--434",
journal = "Nucleic acids symposium series (2004)",
issn = "1746-8272",
publisher = "Oxford University Press",
number = "51",

}

TY - JOUR

T1 - Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine.

AU - Sato, Akira

AU - Miyazaki, Eriko

AU - Satake, Akito

AU - Hiramoto, Akiko

AU - Hiraoka, Osamu

AU - Miyake, Tsuyoshi

AU - Kim, Hye-Sook

AU - Wataya, Yusuke

PY - 2007

Y1 - 2007

N2 - 5-fluoro-2'-deoxyuridine (FUdR) inhibits thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with FUdR. To understand the molecular mechanisms of regulate of two differential cell death necrosis and apoptosis, we identify cell death regulator by using proteome and transcriptome analysis. When compared with the proteome of F28-7 and F28-7-A strain after treated with FUdR, it was found that 5 proteins were up-regulated and 11 proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of necrosis or apoptosis at the proteome and transcriptome levels.

AB - 5-fluoro-2'-deoxyuridine (FUdR) inhibits thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with FUdR. To understand the molecular mechanisms of regulate of two differential cell death necrosis and apoptosis, we identify cell death regulator by using proteome and transcriptome analysis. When compared with the proteome of F28-7 and F28-7-A strain after treated with FUdR, it was found that 5 proteins were up-regulated and 11 proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of necrosis or apoptosis at the proteome and transcriptome levels.

UR - http://www.scopus.com/inward/record.url?scp=42949108752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42949108752&partnerID=8YFLogxK

M3 - Article

C2 - 18029772

AN - SCOPUS:42949108752

SP - 433

EP - 434

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 51

ER -