Proteolytic activation of Vibrio mimicus (Vm) major outer membrane protein haemagglutinin (HA) with Vm-HA/Protease: Implication for understanding bacterial adherence

Munirul Alam, Shin Ichi Miyoshi, Kabir Uddin Ahmed, Nur A. Hasan, Ken Ichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm-HA/protease) and a major outer membrane protein-HA (Vm-OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm-HA/protease and Vm-OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm-OMPHA was confirmed by SDS-PAGE. The proteolytic activation of the native cell-associated Vm-OMPHA by limited proteolysis was also demonstrated in several V. mimicus strains. Proteolytic activation of OMPHA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V. mimicus HAs in the adherence of the bacterium.

Original languageEnglish
Pages (from-to)845-850
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume50
Issue number11
DOIs
Publication statusPublished - Jan 1 2006

Keywords

  • HA/protease
  • Haemagglutinin
  • Outer membrane protein-HA
  • Vibrio mimicus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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