Proteolipid of vacuolar H+-ATPase of Plasmodium falciparum: cDNA cloning, gene organization and complementation of a yeast null mutant

Shouki Yatsushiro, Shinya Taniguchi, Toshihide Mitamura, Hiroshi Omote, Yoshinori Moriyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump, is highly expressed in Plasmodium falciparum, the human malaria parasite. Although V-ATPase-driven proton transport is involved in various physiological processes in the parasite, the overall features of the V-ATPase of P. falciparum, including the gene organization and biogenesis, are far less known. Here, we report cDNA cloning of proteolipid subunit c of P. falciparum, the smallest and most highly hydrophobic subunit of V-ATPase. RT-PCR analysis as well as Northern blotting indicated expression of the proteolipid gene in the parasite cells. cDNA, which encodes a complete reading frame comprising 165 amino acids, was obtained, and its deduced amino acid sequence exhibits 52 and 57% similarity to the yeast and human counterparts, respectively. Southern blot analysis suggested the presence of a single copy of the proteolipid gene, with 5 exons and 4 introns. Upon transfection of the cDNA into a yeast null mutant, the cells became able to grow at neutral pH, accompanied by vesicular accumulation of quinacrine. In contrast, a mutated proteolipid with replacement of glutamate residue 138 with glutamine did not lead to recovery of the growth ability or vesicular accumulation of quinacrine. These results indicated that the cDNA actually encodes the proteolipid of P. falciparum and that the proteolipid is functional in yeast.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalBBA - Biomembranes
Volume1717
Issue number2
DOIs
Publication statusPublished - Nov 30 2005

Fingerprint

Vacuolar Proton-Translocating ATPases
Proteolipids
Cloning
Plasmodium falciparum
Yeast
Organism Cloning
Complementary DNA
Genes
Yeasts
Quinacrine
Parasites
Physiological Phenomena
Amino Acids
Proton Pumps
Reading Frames
Null Lymphocytes
Falciparum Malaria
Southern Blotting
Glutamine
Northern Blotting

Keywords

  • Complementation
  • Malaria
  • Plasmodium falciparum
  • Proteolipid
  • Subunit c
  • V-ATPase
  • Yeast null mutant

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Proteolipid of vacuolar H+-ATPase of Plasmodium falciparum : cDNA cloning, gene organization and complementation of a yeast null mutant. / Yatsushiro, Shouki; Taniguchi, Shinya; Mitamura, Toshihide; Omote, Hiroshi; Moriyama, Yoshinori.

In: BBA - Biomembranes, Vol. 1717, No. 2, 30.11.2005, p. 89-96.

Research output: Contribution to journalArticle

Yatsushiro, Shouki ; Taniguchi, Shinya ; Mitamura, Toshihide ; Omote, Hiroshi ; Moriyama, Yoshinori. / Proteolipid of vacuolar H+-ATPase of Plasmodium falciparum : cDNA cloning, gene organization and complementation of a yeast null mutant. In: BBA - Biomembranes. 2005 ; Vol. 1717, No. 2. pp. 89-96.
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