Protein tyrosine phosphorylation during phytohormone-stimulated cell proliferation in Arabidopsis hypocotyls

Hao Jen Huang, Yu Mei Lin, Dinq Ding Huang, Taku Takahashi, Munetaka Sugiyama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Very little is known about the molecular events triggering differentiated cells to re-enter the cell cycle. We have investigated the possible role of tyrosine phosphorylation in this process with hypocotyl explants of Arabidopsis thaliana. Phytohormone-stimulated cell cycle reactivation in hypocotyls was accompanied by tyrosine phosphorylation of several proteins. Such regulation of the tyrosine phosphorylation in these proteins was not observed in a callus-formation-deficient mutant, srd2, a result which suggests that the induction of tyrosine phosphorylation occurs as a specific event in callus cell proliferation. The promoter activity of cyclin-dependent kinase, CDKA;1, was also examined in phytohormone-stimulated hypocotyls. This study highlighted that protein tyrosine phosphorylation may play an important regulatory role in phytohormone-stimulated cell proliferation.

Original languageEnglish
Pages (from-to)770-775
Number of pages6
JournalPlant and Cell Physiology
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

Fingerprint

Hypocotyl
Plant Growth Regulators
Arabidopsis
plant hormones
hypocotyls
Tyrosine
tyrosine
cell proliferation
phosphorylation
Phosphorylation
Cell Proliferation
protein phosphorylation
Bony Callus
Proteins
proteins
cell cycle
Cell Cycle
CDC2 Protein Kinase
cyclin-dependent kinase
callus formation

Keywords

  • Cell proliferation
  • Cyclin-dependent kinase
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Protein tyrosine phosphorylation during phytohormone-stimulated cell proliferation in Arabidopsis hypocotyls. / Huang, Hao Jen; Lin, Yu Mei; Huang, Dinq Ding; Takahashi, Taku; Sugiyama, Munetaka.

In: Plant and Cell Physiology, Vol. 44, No. 7, 01.07.2003, p. 770-775.

Research output: Contribution to journalArticle

Huang, Hao Jen ; Lin, Yu Mei ; Huang, Dinq Ding ; Takahashi, Taku ; Sugiyama, Munetaka. / Protein tyrosine phosphorylation during phytohormone-stimulated cell proliferation in Arabidopsis hypocotyls. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 7. pp. 770-775.
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