Protein-tyrosine phosphatase α, RPTPα, is a Helicobacter pylori VacA receptor

Kinnosuke Yahiro, Akihiro Wada, Masaaki Nakayama, Takahiro Kimura, Ken ichi Ogushi, Takuro Niidome, Haruhiko Aoyagi, Ken ichi Yoshino, Kazuyoshi Yonezawa, Joel Moss, Toshiya Hirayama

Research output: Contribution to journalArticle

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Abstract

Helicobacter pylori vacuolating cytotoxin, VacA, induces vacuolation, mitochondrial damage, cytochrome c release, and apoptosis of gastric epithelial cells. To detect gastric proteins that serve as VacA receptors, we used VacA co-immunoprecipitation techniques following biotinylation of the cell surface and identified p250, a receptor-like protein-tyrosine phosphatase β (RPTPβ) as a VacA-binding protein (Yahiro, K., Niidome, T., Kimura, M., Hatakeyama, T., Aoyagi, H., Kurazono, H., Imagawa, K., Wada, A., Moss, J., and Hirayama, T. (1999) J. Biol. Chem. 274, 36693-36699). VacA causes vacuolation of G401 cells, a human kidney tumor cell line, although they do not express RPTPβ. By co-immunoprecipitation with VacA, we identified p140 as a potential receptor in those cells. p140 purified by chromatography on a peanut agglutinin affinity matrix contained internal amino acid sequences of RGEENTDYVNASFIDGYRQK and AEGILDVFQTVK, which are identical to those in RPTPα. The peptide mass fingerprinting of p140 by time of flight-MS analysis also supported this identification. Treatment of G401 cells with RPTPα-morpholino antisense oligonucleotide before exposure to toxin inhibited vacuolation. These data suggest that RPTPα acts as a receptor for VacA in G401 cells. Thus, two receptor tyrosine phosphatases, RPTPα and RPTPβ, serve as VacA receptors.

Original languageEnglish
Pages (from-to)19183-19189
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number21
DOIs
Publication statusPublished - May 23 2003
Externally publishedYes

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Receptor-Like Protein Tyrosine Phosphatases
Protein Tyrosine Phosphatases
Phosphatases
Helicobacter pylori
Proteins
Immunoprecipitation
Stomach
Peanut Agglutinin
Biotinylation
Morpholinos
Peptide Mapping
Antisense Oligonucleotides
Cytotoxins
Chromatography
Cytochromes c
Tumor Cell Line
Phosphoric Monoester Hydrolases
Tumors
Amino Acid Sequence
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yahiro, K., Wada, A., Nakayama, M., Kimura, T., Ogushi, K. I., Niidome, T., ... Hirayama, T. (2003). Protein-tyrosine phosphatase α, RPTPα, is a Helicobacter pylori VacA receptor. Journal of Biological Chemistry, 278(21), 19183-19189. https://doi.org/10.1074/jbc.M300117200

Protein-tyrosine phosphatase α, RPTPα, is a Helicobacter pylori VacA receptor. / Yahiro, Kinnosuke; Wada, Akihiro; Nakayama, Masaaki; Kimura, Takahiro; Ogushi, Ken ichi; Niidome, Takuro; Aoyagi, Haruhiko; Yoshino, Ken ichi; Yonezawa, Kazuyoshi; Moss, Joel; Hirayama, Toshiya.

In: Journal of Biological Chemistry, Vol. 278, No. 21, 23.05.2003, p. 19183-19189.

Research output: Contribution to journalArticle

Yahiro, K, Wada, A, Nakayama, M, Kimura, T, Ogushi, KI, Niidome, T, Aoyagi, H, Yoshino, KI, Yonezawa, K, Moss, J & Hirayama, T 2003, 'Protein-tyrosine phosphatase α, RPTPα, is a Helicobacter pylori VacA receptor', Journal of Biological Chemistry, vol. 278, no. 21, pp. 19183-19189. https://doi.org/10.1074/jbc.M300117200
Yahiro, Kinnosuke ; Wada, Akihiro ; Nakayama, Masaaki ; Kimura, Takahiro ; Ogushi, Ken ichi ; Niidome, Takuro ; Aoyagi, Haruhiko ; Yoshino, Ken ichi ; Yonezawa, Kazuyoshi ; Moss, Joel ; Hirayama, Toshiya. / Protein-tyrosine phosphatase α, RPTPα, is a Helicobacter pylori VacA receptor. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 21. pp. 19183-19189.
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AU - Niidome, Takuro

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