Protein translocation within chloroplast is similar in Euglena and higher plants

Junko Inagaki, Yuichi Fujita, Toshiharu Hase, Yasusi Yamamoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

It is currently thought that chloroplasts of higher plants were derived from endosymbiont oxygenic photosynthetic bacteria (primary endosymbiosis), while Euglena, a photosynthetic protista, gained chloroplasts by secondary endosymbiosis (i.e., incorporation of a photosynthetic eukaryote into heterotrophic eukaryotic host). To examine if the protein transport inside chloroplasts is similar between these organisms, we carried out heterologous protein import experiments with Euglena precursor proteins and spinach chloroplasts. The precursor of a 30-kDa subunit of the oxygen-evolving complex (OEC30) from the thylakoid lumen of Euglena chloroplasts contained the N-terminal signal, stroma targeting, and thylakoid transfer domains. Truncated preOEC30s lacking the N-terminal domain were post-translationally imported into spinach chloroplasts, transported into the thylakoid lumen, and processed to a mature protein. These results showed that protein translocations within chloroplasts in Euglena and higher plants are similar and supported the hypothesis that Euglena chloroplasts are derived from the ancestral Chlorophyta. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)436-442
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume277
Issue number2
DOIs
Publication statusPublished - Oct 22 2000

Keywords

  • Chimeric precursor
  • Chloroplast
  • Euglena gracilis
  • Oxygen-evolving complex
  • Photosystem II
  • Protein transport

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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