Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides

Toru Miwa, Ryosei Minoda, Taku Kaitsuka, Momoko Ise, Kazuhito Tomizawa, Eiji Yumoto

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    The mouse otocyst, an anlage of the inner ear, is an attractive experimental target for developing treatment modalities for congenital inner ear diseases and for studying inner ear development. Poly-arginine (6-12 residues) is a cell-penetrating peptide and can be used to deliver cargo into cells. Here, we achieved transutero delivery of enhanced green fluorescent protein (EGFP) fused to a nine-arginine peptide into mouse embryonic otocysts. The EGFP signal was detected both in the lining cells of the otocysts and in their vicinity at 18 h post injection. Mice injected with EGFP fused to a nine-arginine peptide had normal auditory and vestibular functions. These data suggest that protein transduction using poly-arginine may be a useful alternative strategy to commonly used gene delivery methods for delivering therapeutically relevant molecules to the developing inner ear.

    Original languageEnglish
    Pages (from-to)994-999
    Number of pages6
    JournalNeuroReport
    Volume22
    Issue number18
    DOIs
    Publication statusPublished - Dec 21 2011

    Keywords

    • Cell-penetrating peptides
    • otocyst
    • poly-arginine
    • protein transduction

    ASJC Scopus subject areas

    • Neuroscience(all)

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