Abstract
The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 281-287 |
| Number of pages | 7 |
| Journal | Journal of Insect Physiology |
| Volume | 46 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2000 |
| Externally published | Yes |
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Keywords
- Circadian clock
- Crickets
- Optic lobe
- Protein synthesis
ASJC Scopus subject areas
- Insect Science
- Physiology
Cite this
Protein synthesis is a required process for the optic lobe circadian clock in the cricket Gryllus bimaculatus. / Tomioka, Kenji.
In: Journal of Insect Physiology, Vol. 46, No. 3, 03.2000, p. 281-287.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein synthesis is a required process for the optic lobe circadian clock in the cricket Gryllus bimaculatus
AU - Tomioka, Kenji
PY - 2000/3
Y1 - 2000/3
N2 - The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.
AB - The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.
KW - Circadian clock
KW - Crickets
KW - Optic lobe
KW - Protein synthesis
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UR - http://www.scopus.com/inward/citedby.url?scp=0034048325&partnerID=8YFLogxK
U2 - 10.1016/S0022-1910(99)00181-X
DO - 10.1016/S0022-1910(99)00181-X
M3 - Article
AN - SCOPUS:0034048325
VL - 46
SP - 281
EP - 287
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 3
ER -