Protein synthesis is a required process for the optic lobe circadian clock in the cricket Gryllus bimaculatus

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6 Citations (Scopus)

Abstract

The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalJournal of Insect Physiology
Volume46
Issue number3
DOIs
Publication statusPublished - Mar 2000
Externally publishedYes

Fingerprint

optic lobe
Gryllidae
Gryllus bimaculatus
Circadian Clocks
cycloheximide
Cycloheximide
circadian rhythm
protein synthesis
Proteins
compound eyes
Circadian Rhythm
optics
translation (genetics)

Keywords

  • Circadian clock
  • Crickets
  • Optic lobe
  • Protein synthesis

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

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title = "Protein synthesis is a required process for the optic lobe circadian clock in the cricket Gryllus bimaculatus",
abstract = "The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.",
keywords = "Circadian clock, Crickets, Optic lobe, Protein synthesis",
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AU - Tomioka, Kenji

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N2 - The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.

AB - The effects of a translation inhibitor, cycloheximide (CHX), on the circadian neuronal activity rhythm of the optic laminamedulla compound eye complex cultured in vitro were investigated in the cricket Gryllus bimaculatus. When the complex was treated with 10-5 M CHX for 6 h, the rhythm exhibited a marked phase shift. The magnitude and direction of the phase shift were dependent on the phase at which the complex was treated with CHX; phase delays occurred during the late subjective day to early subjective night, whereas phase advances occurred around the late subjective night. Continuous application of CHX abolished circadian rhythms of both the spontaneous neuronal activity and the visually evoked response. However, it abolished neither the spontaneous activity nor the visually evoked response. As washed with fresh medium after CHX treatment, the rhythm soon reappeared and the subsequent phase was clearly correlated to the termination time of the treatment. These results suggest that protein synthesis is also involved in the cricket optic lobe circadian clock, and that the clock-related protein synthesis may be active during the late subjective day to subjective night. (C) 2000 Elsevier Science Ltd.

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KW - Crickets

KW - Optic lobe

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