Protein-protein interaction changes in an archaeal light-signal transduction

Hideki Kandori, Yuki Sudo, Yuji Furutani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Negative phototaxis in Natronomonas pharaonis is initiated by transient interaction changes between photoreceptor and transducer. pharaonis phoborhodopsin (ppR; also called pharaonis sensory rhodopsin II, psR-II) and the cognate transducer protein, pHtrII, form a tight 2:2 complex in the unphotolyzed state, and the interaction is somehow altered during the photocycle of ppR. We have studied the signal transduction mechanism in the ppR/pHtrII system by means of low-temperature Fourier-transform infrared (FTIR) spectroscopy. In the paper, spectral comparison in the absence and presence of pHtrII provided fruitful information in atomic details, where vibrational bands were identified by the use of isotope-labeling and site-directed mutagenesis. From these studies, we established the two pathways of light-signal conversion from the receptor to the transducer; (i) from Lys205 (retinal) of ppR to Asn74 of pHtrII through Thr204 and Tyr199, and (ii) from Lys205 of ppR to the cytoplasmic loop region of pHtrII that links Gly83.

Original languageEnglish
Article number424760
JournalJournal of Biomedicine and Biotechnology
Volume2010
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Light Signal Transduction
Signal transduction
Transducers
Signal Transduction
Sensory Rhodopsins
Isotope Labeling
Mutagenesis
Proteins
Fourier Transform Infrared Spectroscopy
Site-Directed Mutagenesis
Isotopes
Labeling
Light
Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Protein-protein interaction changes in an archaeal light-signal transduction. / Kandori, Hideki; Sudo, Yuki; Furutani, Yuji.

In: Journal of Biomedicine and Biotechnology, Vol. 2010, 424760, 2010.

Research output: Contribution to journalArticle

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