Protein imaging of CCN2 AND CCN3 in living cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recent progress in molecular imaging technology has had a strong impact on improving our understanding of molecular translocation, receptor internalization, and interactions in living cells. The protocol in this chapter introduces an optimized technique for intracellular localization of CCN2 and CCN3 in live cells, one using GFP-tagged CCN2 and Halo-tagged CCN3.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages211-215
Number of pages5
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

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Keywords

  • GFP
  • Halo
  • Molecular imaging
  • Protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hattori, T., Hoshijima, M., & Takigawa, M. (2017). Protein imaging of CCN2 AND CCN3 in living cells. In Methods in Molecular Biology (Vol. 1489, pp. 211-215). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_20