Protein adsorption on hydroxyapatite nanosensors with different crystal sizes studied in situ by a quartz crystal microbalance with the dissipation method

Toshiyuki Ikoma, Motohiro Tagaya, Nobutaka Hanagata, Tomohiko Yoshioka, Dinko Chakarov, Bengt Kasemo, Junzo Tanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Hydroxyapatite (HAp) nanocrystals with different crystal sizes were deposited by the electrophoretic deposition method on the gold surface of a quartz crystal microbalance with a dissipation probe. The nanosensors formed this way were used to elucidate the adsorption mechanism of proteins with a similar pI value. The crystal sizes and the area of the a-plane affected only the adsorption amount of human serum albumin, but not that of bovine plasma fibrinogen. The viscoelastic property, ΔD/Δf, of each absorbed layer on the nanosensors was almost constant. The protein adsorption mechanism can be explained as follows: the dissociated carboxyl groups (negative charge) of albumin were interacted with calcium ions and the hydrated amine groups (positive charge) at the αC domain of fibrinogen were with phosphate ions on the HAp surface.

Original languageEnglish
Pages (from-to)1125-1128
Number of pages4
JournalJournal of the American Ceramic Society
Volume92
Issue number5
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Protein adsorption on hydroxyapatite nanosensors with different crystal sizes studied in situ by a quartz crystal microbalance with the dissipation method'. Together they form a unique fingerprint.

  • Cite this