Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique

Tomohiko Yoshioka, T. Ikoma, A. Monkawa, T. Tonegawa, D. Chakarov, B. Kasemo, N. Hanagata, J. Tanaka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Real time adsorption behaviors of six proteins with different isoelectric points on hydroxyapatite (HAp) nanocrystal surfaces have been investigated by using HAp sensors for quartz crystal microbalance with dissipation technique (QCM-D). The dissipation (D)) - frequency (f) plots clearly showed that the different types of protein adsorption behaviors; the D-f plots of acidic proteins lie on one straight line with a constant slope under all initial protein concentrations, while those of neutral and basic proteins lie on two straight lines with different slopes. The acidic proteins formed a monolayer, while the neutral and basic proteins could cause conformational changes with the adsorbed amount of proteins. The QCM-D technique with novel HAp nanocrystal sensor is useful for the liquid phase changes of proteins on the surface.

Original languageEnglish
Pages (from-to)1119-1122
Number of pages4
JournalKey Engineering Materials
Volume361-363 II
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Quartz crystal microbalances
Durapatite
Hydroxyapatite
Proteins
Adsorption
Crystals
Nanocrystals
Sensors
Monolayers
Liquids

Keywords

  • Hydroxyapatite (HAp)
  • Nanocrystal
  • Protein adsorption
  • QCM-D

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Yoshioka, T., Ikoma, T., Monkawa, A., Tonegawa, T., Chakarov, D., Kasemo, B., ... Tanaka, J. (2008). Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique. Key Engineering Materials, 361-363 II, 1119-1122.

Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique. / Yoshioka, Tomohiko; Ikoma, T.; Monkawa, A.; Tonegawa, T.; Chakarov, D.; Kasemo, B.; Hanagata, N.; Tanaka, J.

In: Key Engineering Materials, Vol. 361-363 II, 2008, p. 1119-1122.

Research output: Contribution to journalArticle

Yoshioka, T, Ikoma, T, Monkawa, A, Tonegawa, T, Chakarov, D, Kasemo, B, Hanagata, N & Tanaka, J 2008, 'Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique', Key Engineering Materials, vol. 361-363 II, pp. 1119-1122.
Yoshioka T, Ikoma T, Monkawa A, Tonegawa T, Chakarov D, Kasemo B et al. Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique. Key Engineering Materials. 2008;361-363 II:1119-1122.
Yoshioka, Tomohiko ; Ikoma, T. ; Monkawa, A. ; Tonegawa, T. ; Chakarov, D. ; Kasemo, B. ; Hanagata, N. ; Tanaka, J. / Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique. In: Key Engineering Materials. 2008 ; Vol. 361-363 II. pp. 1119-1122.
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