Protein adsorption and subsequent fibroblasts adhesion on hydroxyapatite nanocrystals

Motohiro Tagaya, Toshiyuki Ikoma, Taro Takemura, Nobutaka Hanagata, Tomohiko Yoshioka, Junzo Tanaka

Research output: Contribution to journalConference article

6 Citations (Scopus)

Abstract

Quartz crystal microbalance with dissipation (QCM-D) technique was employed for protein adsorption and subsequent fibroblast adhesion on hydroxyapatite (HAp) nanocrystals. The pre-adsorption of three proteins (albumin (BSA) or fibronectin (Fn) or collagen (Col)) and subsequent adsorption of fetal bovine serum (FBS), and the adhesion of fibroblasts on the surface were in situ monitored, and evaluated with the frequency shift (Δf) and dissipation energy shift (ΔD), and the viscoelastic change as ΔD-Δf plot. The Col adsorption showed larger Δf and ΔD values compared with BSA or Fn adsorption, and the subsequent FBS adsorption depended on the pre-adsorbed proteins. The ΔD-Δf plot of the cell adhesion also showed the different behaviour on the surfaces, indicating the process affected by cell-protein interactions. The confocal laser scanning microscope images of adherent cells showed the different morphology and pseudopod on the surfaces. The cells adhered on the surfaces modified with Fn and Col had the uniaxially expanded shape with fibrous pseudopods, while those modified with BSA had round shape. The different cell-protein interaction would cause the arrangement of extracellular matrix and cytoskeleton changes at the interfaces.

Original languageEnglish
Article number192009
JournalIOP Conference Series: Materials Science and Engineering
Volume18
Issue numberSYMPOSIUM 13
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event3rd International Congress on Ceramics, ICC3 - Osaka, Japan
Duration: Nov 14 2010Nov 18 2010

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)

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