Protective role of heme oxygenase 1 in the intestinal tissue injury in hemorrhagic shock in rats

Kazuyoshi Inoue, Toru Takahashi, Kenji Uehara, Hiroko Shimuzu, Kana Ido, Hiroshi Morimatsu, Emiko Omori, Hiroshi Katayama, Reiko Akagi, Kiyoshi Morita

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Heme oxygenase (HO) 1 is inducible by a variety of oxidative stress and is thought to play an important role in the protection of tissues from oxidative injuries. Because hemorrhagic shock (HS) is an oxidative stress that results in tissue injury, we examined in this study the role of HO-1 induction in intestinal tissue injuries in a rat model of HS. The levels of HO-1 were significantly increased after HS both at transcriptional and protein levels in mucosal epithelial cells in the duodenum, jejunum, and colon, whereas their expression in the ileum was hardly detectable and not increased at all by the treatment. In contrast, HS-induced mucosal inflammation and apoptotic cell death in the duodenum, jejunum, and colon were far less than those observed in ileum as judged by the levels of expression of TNF-α, iNOS, activated caspase 3, and Bcl-2. Of note, inhibition of HO activity by tin-mesoporphyrin resulted in an aggravation of HS-induced tissue inflammation and apoptotic cell death. These findings indicate that HO-1 expression in the intestine is regulated in a highly site-specific manner after HS, and that HO-1 induction plays a fundamental role in protecting mucosal cells of the intestine from oxidative damages induced by HS.

Original languageEnglish
Pages (from-to)252-261
Number of pages10
JournalShock
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Heme Oxygenase-1
Hemorrhagic Shock
Wounds and Injuries
Jejunum
Ileum
Duodenum
Intestines
Colon
Oxidative Stress
Cell Death
Inflammation
Heme Oxygenase (Decyclizing)
Caspase 3
Epithelial Cells

Keywords

  • Apoptosis
  • Heme
  • Inflammation
  • Oxidative stress
  • Tin-mesoporphyrin

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Protective role of heme oxygenase 1 in the intestinal tissue injury in hemorrhagic shock in rats. / Inoue, Kazuyoshi; Takahashi, Toru; Uehara, Kenji; Shimuzu, Hiroko; Ido, Kana; Morimatsu, Hiroshi; Omori, Emiko; Katayama, Hiroshi; Akagi, Reiko; Morita, Kiyoshi.

In: Shock, Vol. 29, No. 2, 02.2008, p. 252-261.

Research output: Contribution to journalArticle

Inoue, K, Takahashi, T, Uehara, K, Shimuzu, H, Ido, K, Morimatsu, H, Omori, E, Katayama, H, Akagi, R & Morita, K 2008, 'Protective role of heme oxygenase 1 in the intestinal tissue injury in hemorrhagic shock in rats', Shock, vol. 29, no. 2, pp. 252-261. https://doi.org/10.1097/shk.0b013e3180cab913
Inoue, Kazuyoshi ; Takahashi, Toru ; Uehara, Kenji ; Shimuzu, Hiroko ; Ido, Kana ; Morimatsu, Hiroshi ; Omori, Emiko ; Katayama, Hiroshi ; Akagi, Reiko ; Morita, Kiyoshi. / Protective role of heme oxygenase 1 in the intestinal tissue injury in hemorrhagic shock in rats. In: Shock. 2008 ; Vol. 29, No. 2. pp. 252-261.
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