Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

Naoya Oohara, Masanori Matsuoka, Hiroko Nomaguchi, Mariko Naito, Takeshi Yamada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

Original languageEnglish
Pages (from-to)1906-1910
Number of pages5
JournalVaccine
Volume19
Issue number15-16
DOIs
Publication statusPublished - Feb 28 2001
Externally publishedYes

Fingerprint

Mycobacterium leprae
Mycobacterium Infections
Mycobacterium bovis
Bacillus (bacteria)
Bacillus
antigens
Antigens
mice
infection
mycobacterial diseases
immunization
Foot
Immunization

Keywords

  • Ag85
  • BCG
  • Leprosy
  • Recombinant
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates. / Oohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko; Naito, Mariko; Yamada, Takeshi.

In: Vaccine, Vol. 19, No. 15-16, 28.02.2001, p. 1906-1910.

Research output: Contribution to journalArticle

@article{ab6ff0c62d1345409af00dec0ab56665,
title = "Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Gu{\'e}rin over-producing three putative protective antigen candidates",
abstract = "The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Gu{\'e}rin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.",
keywords = "Ag85, BCG, Leprosy, Recombinant, Vaccine",
author = "Naoya Oohara and Masanori Matsuoka and Hiroko Nomaguchi and Mariko Naito and Takeshi Yamada",
year = "2001",
month = "2",
day = "28",
doi = "10.1016/S0264-410X(00)00439-4",
language = "English",
volume = "19",
pages = "1906--1910",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "15-16",

}

TY - JOUR

T1 - Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

AU - Oohara, Naoya

AU - Matsuoka, Masanori

AU - Nomaguchi, Hiroko

AU - Naito, Mariko

AU - Yamada, Takeshi

PY - 2001/2/28

Y1 - 2001/2/28

N2 - The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

AB - The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

KW - Ag85

KW - BCG

KW - Leprosy

KW - Recombinant

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=0035961592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035961592&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(00)00439-4

DO - 10.1016/S0264-410X(00)00439-4

M3 - Article

C2 - 11228360

AN - SCOPUS:0035961592

VL - 19

SP - 1906

EP - 1910

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 15-16

ER -