Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model

Soumik Barman, Hemanta Koley, Thandavarayan Ramamurthy, Manoj Kumar Chakrabarti, Sumio Shinoda, Gopinath Balakrish Nair, Yoshifumi Takeda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains (Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1×107 of each serogroup/serotype of heat-killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1×109 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat-killed cells of the six Shigella serogroups/serotypes studied would be a possible broad-spectrum candidate vaccine against shigellosis.

Original languageEnglish
Pages (from-to)762-771
Number of pages10
JournalMicrobiology and Immunology
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

Fingerprint

Shigella
Colitis
Immunity
Immunization
Guinea Pigs
Hot Temperature
Shigella flexneri
Immunoglobulin A
Lipopolysaccharides
Serogroup
Shigella boydii
Shigella dysenteriae
Shigella sonnei
Bacillary Dysentery
Therapeutic Irrigation
Vaccines
Immunoglobulin G

Keywords

  • Heat-killed Shigella cells
  • Hexavalent Shigella immunogen
  • Protective immunity
  • Shigellosis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Barman, S., Koley, H., Ramamurthy, T., Chakrabarti, M. K., Shinoda, S., Nair, G. B., & Takeda, Y. (2013). Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model. Microbiology and Immunology, 57(11), 762-771. https://doi.org/10.1111/1348-0421.12095

Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model. / Barman, Soumik; Koley, Hemanta; Ramamurthy, Thandavarayan; Chakrabarti, Manoj Kumar; Shinoda, Sumio; Nair, Gopinath Balakrish; Takeda, Yoshifumi.

In: Microbiology and Immunology, Vol. 57, No. 11, 11.2013, p. 762-771.

Research output: Contribution to journalArticle

Barman, S, Koley, H, Ramamurthy, T, Chakrabarti, MK, Shinoda, S, Nair, GB & Takeda, Y 2013, 'Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model', Microbiology and Immunology, vol. 57, no. 11, pp. 762-771. https://doi.org/10.1111/1348-0421.12095
Barman, Soumik ; Koley, Hemanta ; Ramamurthy, Thandavarayan ; Chakrabarti, Manoj Kumar ; Shinoda, Sumio ; Nair, Gopinath Balakrish ; Takeda, Yoshifumi. / Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model. In: Microbiology and Immunology. 2013 ; Vol. 57, No. 11. pp. 762-771.
@article{dd3c6b6a51c04162b6b770afeb194412,
title = "Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model",
abstract = "The protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains (Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1×107 of each serogroup/serotype of heat-killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1×109 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat-killed cells of the six Shigella serogroups/serotypes studied would be a possible broad-spectrum candidate vaccine against shigellosis.",
keywords = "Heat-killed Shigella cells, Hexavalent Shigella immunogen, Protective immunity, Shigellosis",
author = "Soumik Barman and Hemanta Koley and Thandavarayan Ramamurthy and Chakrabarti, {Manoj Kumar} and Sumio Shinoda and Nair, {Gopinath Balakrish} and Yoshifumi Takeda",
year = "2013",
month = "11",
doi = "10.1111/1348-0421.12095",
language = "English",
volume = "57",
pages = "762--771",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "11",

}

TY - JOUR

T1 - Protective immunity by oral immunization with heat-killed Shigella strains in a guinea pig colitis model

AU - Barman, Soumik

AU - Koley, Hemanta

AU - Ramamurthy, Thandavarayan

AU - Chakrabarti, Manoj Kumar

AU - Shinoda, Sumio

AU - Nair, Gopinath Balakrish

AU - Takeda, Yoshifumi

PY - 2013/11

Y1 - 2013/11

N2 - The protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains (Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1×107 of each serogroup/serotype of heat-killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1×109 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat-killed cells of the six Shigella serogroups/serotypes studied would be a possible broad-spectrum candidate vaccine against shigellosis.

AB - The protective efficacy of and immune response to heat-killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains (Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1×107 of each serogroup/serotype of heat-killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1×109 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat-killed cells of the six Shigella serogroups/serotypes studied would be a possible broad-spectrum candidate vaccine against shigellosis.

KW - Heat-killed Shigella cells

KW - Hexavalent Shigella immunogen

KW - Protective immunity

KW - Shigellosis

UR - http://www.scopus.com/inward/record.url?scp=84887764371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887764371&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12095

DO - 10.1111/1348-0421.12095

M3 - Article

C2 - 24033533

AN - SCOPUS:84887764371

VL - 57

SP - 762

EP - 771

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 11

ER -