Protective effects of nicergoline against hydrogen peroxide toxicity in rat neuronal cell line

Emi Iwata, Ikuko Miyazaki, Masato Asanuma, Azusa Iida, Norio Ogawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We examined the effects of nicergoline on hydrogen peroxide (H2O2)- induced neurotoxicity in cultured rat neuronal cell line (B50). H2O2 induced death of B50 cells in a dose-dependent manner. The H2O2-induced neuronal cell death was significantly decreased in B50 cells maintained in the presence of nicergoline. We compared the levels of antioxidants (glutathione, catalase and superoxide dismutase) in nicergoline-treated and untreated B50 cells. Lipid peroxidation products (thiobarbituric acid reactive substances, TBARS) levels were also measured. Cultures treated with nicergoline had higher levels of catalase activity. TBARS level was significantly lower in nicergoline-treated cells than in untreated cells. Our results suggest that nicergoline may induce the up-regulation of intracellular antioxidant defences and protect the neuronal cells against oxidative stress.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalNeuroscience Letters
Volume251
Issue number1
DOIs
Publication statusPublished - Jul 17 1998

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Keywords

  • Antioxidant
  • Cell culture
  • Hydrogen peroxide
  • Neuroprotection
  • Neurotoxicity
  • Nicergoline
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)

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