Protective effect of oren-gedoku-to against induction of neuronal death by transient cerebral ischemia in the C57BL/6 mouse

Yoichi Kondo, Fumio Kondo, Masato Asanuma, Ken Ichi Tanaka, Norio Ogawa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We examined the neuroprotective effects of oren-gedoku-to (TJ15), a herbal medicine, after transient forebrain ischemia. Transient forebrain ischemia was induced by occlusion of both common carotid arteries for 15 min in C57BL/6 mice treated with TJ15. In the control ischemic group without TJ15 treatment, histologic examination of brain tissue collected seven days after reperfusion showed death of pyramidal cells in CA2-3 area of the hippocampus, unilaterally or bilaterally. In mice treated with oral TJ15 (845 mg/kg/day) for five weeks, the frequency of ischemic neuronal death was significantly lower. Immunohistochemistry for Cu/Zn-superoxide dismutase (Cu/Zn-SOD) showed strongly reactive astrocytes in the hippocampus of ischemic mice treated with TJ15. Damage to nerve cells by free radicals plays an important role in the induction of neuronal death by ischemia-reperfusion injury. Our results suggest that TJ15 protects against ischemic neuronal death by increasing the expression of Cu/Zn-SOD and suggest that oren-gedoku-to reduces the exposure of hippocampal neurons to oxidative stress.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalNeurochemical Research
Volume25
Issue number2
DOIs
Publication statusPublished - 2000

Fingerprint

Transient Ischemic Attack
Inbred C57BL Mouse
Prosencephalon
Neurons
Hippocampus
Ischemia
Oxidative stress
oren gedoku to
Herbal Medicine
Pyramidal Cells
Common Carotid Artery
Neuroprotective Agents
Reperfusion Injury
Astrocytes
Reperfusion
Free Radicals
Brain
Oxidative Stress
Immunohistochemistry
Cells

Keywords

  • C57BL/6 mice
  • Cu/Zn-SOD
  • TJ-15
  • Transient forebrain ischemia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Protective effect of oren-gedoku-to against induction of neuronal death by transient cerebral ischemia in the C57BL/6 mouse. / Kondo, Yoichi; Kondo, Fumio; Asanuma, Masato; Tanaka, Ken Ichi; Ogawa, Norio.

In: Neurochemical Research, Vol. 25, No. 2, 2000, p. 205-209.

Research output: Contribution to journalArticle

Kondo, Yoichi ; Kondo, Fumio ; Asanuma, Masato ; Tanaka, Ken Ichi ; Ogawa, Norio. / Protective effect of oren-gedoku-to against induction of neuronal death by transient cerebral ischemia in the C57BL/6 mouse. In: Neurochemical Research. 2000 ; Vol. 25, No. 2. pp. 205-209.
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