Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation

Suyun Shi, Gang Wang, Yading Wang, Lingang Zhang, Lixin Zhang

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

The response of bean leaves to UV-B radiation was extensively investigated. UV-B radiation caused increase of ion leakage, loss of chlorophyll, and decrease of the maximum efficiency of PSII photochemistry (Fv/F m) and the quantum yield of PSII electron transport (ΦPSII) of bean leaves. H2O2 contents and the extent of thylakoid membrane protein oxidation increased, indicated by the decrease of thiol contents and the increase of carbonyl contents with the duration of UV-B radiation. Addition of sodium nitroprusside, a nitric oxide (NO) donor, can partially alleviate UV-B induced decrease of chlorophyll contents, Fv/Fm and ΦPSII. Moreover, the oxidative damage to the thylakoid membrane was alleviated by NO. The potassium salt of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, a specific NO scavenger, arrested NO mediated protective effects against UV-B induced oxidative damage. Incubation of thylakoid membrane with increasing H2O2 concentrations showed a progressive enhancement in carbonyl contents. H2O2 contents were decreased in the presence of NO under UV-B radiation through increased activities of superoxide dismutases, ascorbate peroxidases, and catalases. Taken together, the results suggest that NO can effectively protect plants from UV-B damage mostly probably mediated by enhanced activities of antioxidant enzymes.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalNitric Oxide - Biology and Chemistry
Volume13
Issue number1
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Oxidative stress
Nitric Oxide
Oxidative Stress
Radiation
Thylakoids
Chlorophyll
Thylakoid Membrane Proteins
Ascorbate Peroxidases
Membranes
Photochemistry
Nitric Oxide Donors
Photochemical reactions
Nitroprusside
Quantum yield
Electron Transport
Sulfhydryl Compounds
Catalase
Superoxide Dismutase
Potassium
Salts

Keywords

  • Hydrogen peroxide
  • Nitric oxide
  • Oxidative damage
  • Thylakoid membrane
  • UV-B

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation. / Shi, Suyun; Wang, Gang; Wang, Yading; Zhang, Lingang; Zhang, Lixin.

In: Nitric Oxide - Biology and Chemistry, Vol. 13, No. 1, 08.2005, p. 1-9.

Research output: Contribution to journalArticle

Shi, Suyun ; Wang, Gang ; Wang, Yading ; Zhang, Lingang ; Zhang, Lixin. / Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation. In: Nitric Oxide - Biology and Chemistry. 2005 ; Vol. 13, No. 1. pp. 1-9.
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