Abstract
Nitric oxide (NO) is a key molecule involved in many physiological processes. To characterize its roles in the tolerance of Arabidopsis thaliana to ultraviolet-B (UV-B), we investigated the effect of a reduced endogenous NO level on oxidative damage to wild-type and mutant (Atnoa1) plants. Under irradiation, hydrogen peroxide was accumulated more in mutant leaves than in the wild type. However, the amounts of UV-B-absorbing compounds (flavonoids and anthocyanin) and the activities of two antioxidant enzymes-catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11)-were lower in leaves of the former. Supplementing with sodium nitroprusside, an NO donor, could alleviate the oxidative damage to mutant leaves by increasing flavonoid and anthocyanin contents and enzyme activities. In comparison, an inhibitor of nitric oxide synthase, had the opposite effects on oxidation resistance in wild-type leaves. All these results suggest that nitric oxide acts as a signal for an active oxygen-scavenging system that protects plants from oxidative stress induced by UV-B irradiation.
| Original language | English |
|---|---|
| Pages (from-to) | 135-140 |
| Number of pages | 6 |
| Journal | Journal of Plant Biology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 2009 |
| Externally published | Yes |
Fingerprint
Keywords
- Nitric oxide
- Oxidative damage
- Ultraviolet-B
ASJC Scopus subject areas
- Plant Science
Cite this
Protective effect of nitric oxide against oxidative damage in arabidopsis leaves under ultraviolet-B irradiation. / Zhang, Lingang; Zhou, Shuo; Xuan, Yi; Sun, Miao; Zhao, Liqun.
In: Journal of Plant Biology, Vol. 52, No. 2, 04.2009, p. 135-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective effect of nitric oxide against oxidative damage in arabidopsis leaves under ultraviolet-B irradiation
AU - Zhang, Lingang
AU - Zhou, Shuo
AU - Xuan, Yi
AU - Sun, Miao
AU - Zhao, Liqun
PY - 2009/4
Y1 - 2009/4
N2 - Nitric oxide (NO) is a key molecule involved in many physiological processes. To characterize its roles in the tolerance of Arabidopsis thaliana to ultraviolet-B (UV-B), we investigated the effect of a reduced endogenous NO level on oxidative damage to wild-type and mutant (Atnoa1) plants. Under irradiation, hydrogen peroxide was accumulated more in mutant leaves than in the wild type. However, the amounts of UV-B-absorbing compounds (flavonoids and anthocyanin) and the activities of two antioxidant enzymes-catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11)-were lower in leaves of the former. Supplementing with sodium nitroprusside, an NO donor, could alleviate the oxidative damage to mutant leaves by increasing flavonoid and anthocyanin contents and enzyme activities. In comparison, an inhibitor of nitric oxide synthase, had the opposite effects on oxidation resistance in wild-type leaves. All these results suggest that nitric oxide acts as a signal for an active oxygen-scavenging system that protects plants from oxidative stress induced by UV-B irradiation.
AB - Nitric oxide (NO) is a key molecule involved in many physiological processes. To characterize its roles in the tolerance of Arabidopsis thaliana to ultraviolet-B (UV-B), we investigated the effect of a reduced endogenous NO level on oxidative damage to wild-type and mutant (Atnoa1) plants. Under irradiation, hydrogen peroxide was accumulated more in mutant leaves than in the wild type. However, the amounts of UV-B-absorbing compounds (flavonoids and anthocyanin) and the activities of two antioxidant enzymes-catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11)-were lower in leaves of the former. Supplementing with sodium nitroprusside, an NO donor, could alleviate the oxidative damage to mutant leaves by increasing flavonoid and anthocyanin contents and enzyme activities. In comparison, an inhibitor of nitric oxide synthase, had the opposite effects on oxidation resistance in wild-type leaves. All these results suggest that nitric oxide acts as a signal for an active oxygen-scavenging system that protects plants from oxidative stress induced by UV-B irradiation.
KW - Nitric oxide
KW - Oxidative damage
KW - Ultraviolet-B
UR - http://www.scopus.com/inward/record.url?scp=70349432548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349432548&partnerID=8YFLogxK
U2 - 10.1007/s12374-009-9013-2
DO - 10.1007/s12374-009-9013-2
M3 - Article
VL - 52
SP - 135
EP - 140
JO - Journal of Plant Biology
JF - Journal of Plant Biology
SN - 1226-9239
IS - 2
ER -