Protective effect of nitric oxide against oxidative damage in arabidopsis leaves under ultraviolet-B irradiation

Lingang Zhang, Shuo Zhou, Yi Xuan, Miao Sun, Liqun Zhao

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Nitric oxide (NO) is a key molecule involved in many physiological processes. To characterize its roles in the tolerance of Arabidopsis thaliana to ultraviolet-B (UV-B), we investigated the effect of a reduced endogenous NO level on oxidative damage to wild-type and mutant (Atnoa1) plants. Under irradiation, hydrogen peroxide was accumulated more in mutant leaves than in the wild type. However, the amounts of UV-B-absorbing compounds (flavonoids and anthocyanin) and the activities of two antioxidant enzymes-catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11)-were lower in leaves of the former. Supplementing with sodium nitroprusside, an NO donor, could alleviate the oxidative damage to mutant leaves by increasing flavonoid and anthocyanin contents and enzyme activities. In comparison, an inhibitor of nitric oxide synthase, had the opposite effects on oxidation resistance in wild-type leaves. All these results suggest that nitric oxide acts as a signal for an active oxygen-scavenging system that protects plants from oxidative stress induced by UV-B irradiation.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalJournal of Plant Biology
Volume52
Issue number2
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

nitric oxide
protective effect
ultraviolet radiation
irradiation
Arabidopsis
ascorbate peroxidase
mutants
anthocyanins
leaves
catalase
flavonoids
nitric oxide synthase
hydrogen peroxide
oxidative stress
Arabidopsis thaliana
enzyme activity
oxidation
oxygen
antioxidants
enzymes

Keywords

  • Nitric oxide
  • Oxidative damage
  • Ultraviolet-B

ASJC Scopus subject areas

  • Plant Science

Cite this

Protective effect of nitric oxide against oxidative damage in arabidopsis leaves under ultraviolet-B irradiation. / Zhang, Lingang; Zhou, Shuo; Xuan, Yi; Sun, Miao; Zhao, Liqun.

In: Journal of Plant Biology, Vol. 52, No. 2, 04.2009, p. 135-140.

Research output: Contribution to journalArticle

Zhang, Lingang ; Zhou, Shuo ; Xuan, Yi ; Sun, Miao ; Zhao, Liqun. / Protective effect of nitric oxide against oxidative damage in arabidopsis leaves under ultraviolet-B irradiation. In: Journal of Plant Biology. 2009 ; Vol. 52, No. 2. pp. 135-140.
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