Protective effect of montmorillonite on plasmid DNA in oral gene delivery into small intestine

Masaya Kawase, Yuka Hayashi, Fumie Kinoshita, Eiji Yamato, Jun Ichi Miyazaki, Junji Yamakawa, Tomoyuki Ishida, Manabu Tamura, Kiyohito Yagi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Montmorillonite, a bioinert clay mineral, was examined as a novel vector for an oral gene-delivery system. The complex of montmorillonite and plasmid DNA encoding the enhanced green fluorescent protein (EGFP) gene was prepared at various weight ratios, and then transfected into cultured intestinal epithelial cells (IEC-6) in vitro. The EGFP gene was clearly transcribed when the transfection was performed using the montmorillonite-plasmid complex at a weight ratio of 0.05:1. In contrast, no gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) analysis when the transfection was performed with naked plasmid. Various plasmid preparations were given orally to mice, and the gene expression in the stomach and small intestine was examined by RT-PCR. Although no gene expression was detected in the mice receiving an oral administration of naked plasmid or polyethyleneimine-plasmid complex, the EGFP gene complexed with montmorillonite was expressed in the small intestine. These results indicate that montmorillonite protected the plasmid DNA from the acidic environment in the stomach and DNA-degrading enzymes in the intestine, and successfully delivered it into cells of the small intestine.

Original languageEnglish
Pages (from-to)2049-2051
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number12
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Bentonite
Small Intestine
Plasmids
DNA
Genes
Gene Expression
Reverse Transcription
Transfection
Stomach
Polyethyleneimine
Weights and Measures
Gene Transfer Techniques
Polymerase Chain Reaction
Intestines
Minerals
Oral Administration
Epithelial Cells
Enzymes

Keywords

  • Clay
  • Gene therapy
  • Montmorillonite
  • Non-viral vector
  • Oral gene delivery

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Protective effect of montmorillonite on plasmid DNA in oral gene delivery into small intestine. / Kawase, Masaya; Hayashi, Yuka; Kinoshita, Fumie; Yamato, Eiji; Miyazaki, Jun Ichi; Yamakawa, Junji; Ishida, Tomoyuki; Tamura, Manabu; Yagi, Kiyohito.

In: Biological and Pharmaceutical Bulletin, Vol. 27, No. 12, 12.2004, p. 2049-2051.

Research output: Contribution to journalArticle

Kawase, M, Hayashi, Y, Kinoshita, F, Yamato, E, Miyazaki, JI, Yamakawa, J, Ishida, T, Tamura, M & Yagi, K 2004, 'Protective effect of montmorillonite on plasmid DNA in oral gene delivery into small intestine', Biological and Pharmaceutical Bulletin, vol. 27, no. 12, pp. 2049-2051. https://doi.org/10.1248/bpb.27.2049
Kawase, Masaya ; Hayashi, Yuka ; Kinoshita, Fumie ; Yamato, Eiji ; Miyazaki, Jun Ichi ; Yamakawa, Junji ; Ishida, Tomoyuki ; Tamura, Manabu ; Yagi, Kiyohito. / Protective effect of montmorillonite on plasmid DNA in oral gene delivery into small intestine. In: Biological and Pharmaceutical Bulletin. 2004 ; Vol. 27, No. 12. pp. 2049-2051.
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