Protective effect of carbon monoxide inhalation on lung injury after hemorrhagic shock/resuscitation in rats

Fumitoshi Kanagawa, Toru Takahashi, Kazuyoshi Inoue, Hiroko Shimizu, Emiko Omori, Hiroshi Morimatsu, Shigeru Maeda, Hiroshi Katayama, Atsunori Nakao, Kiyoshi Morita

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Hemorrhagic shock and resuscitation (HSR) induces pulmonary inflammation that leads to acute lung injury. Carbon monoxide (CO), a by-product of heme catalysis, was shown to have potent cytoprotective and anti-inflammatory effects. The aim of this study was to examine the effects of CO inhalation at low concentration on lung injury induced by HSR in rats. Methods: Rats were subjected to HSR by bleeding to achieve mean arterial pressure of 30 mm Hg for 60 minutes followed by resuscitation with shed blood and saline as needed to restore blood pressure. HSR animals were either maintained in room air or were exposed to CO at 250 ppm for 1 hour before and 3 hours after HSR. Results: HSR caused an increase in the DNA binding activity of nuclear factor-κB and activator protein-1 in the lung followed by the up-regulation of pulmonary gene expression of tumor necrosis factor-α, inducible nitric oxide synthase, and interleukin (IL)-10. HSR also resulted in an increase in myeloperoxidase activity and wet weight to dry weight ratio in the lung, and more prominent histopathologic changes including congestion, edema, cellular infiltration, and hemorrhage. In contrast, CO inhalation significantly ameliorated these inflammatory events as judged by fewer histologic changes, less up-regulation of inflammatory mediators, and less activation of nuclear factor-κB and activator protein-1. Interestingly, the protective effects against lung injury afforded by CO were associated with further increases in mRNA expression of IL-10 in the lung. Conclusions: These findings suggest that inhaled CO at a low concentration ameliorated HSR-induced lung injury and attenuated inflammatory cascades by up-regulation of anti-inflammatory IL-10.

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume69
Issue number1
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Hemorrhagic Shock
Lung Injury
Carbon Monoxide
Resuscitation
Inhalation
Interleukin-10
Lung
Up-Regulation
Transcription Factor AP-1
Anti-Inflammatory Agents
Hemorrhage
Weights and Measures
Acute Lung Injury
Gene Expression Regulation
Nitric Oxide Synthase Type II
Catalysis
Heme
Peroxidase
Edema
Pneumonia

Keywords

  • Activator protein-1
  • Acute lung injury
  • Carbon monoxide
  • Inflammation
  • Interleukin-10
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

Cite this

Protective effect of carbon monoxide inhalation on lung injury after hemorrhagic shock/resuscitation in rats. / Kanagawa, Fumitoshi; Takahashi, Toru; Inoue, Kazuyoshi; Shimizu, Hiroko; Omori, Emiko; Morimatsu, Hiroshi; Maeda, Shigeru; Katayama, Hiroshi; Nakao, Atsunori; Morita, Kiyoshi.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 69, No. 1, 07.2010, p. 185-194.

Research output: Contribution to journalArticle

Kanagawa, Fumitoshi ; Takahashi, Toru ; Inoue, Kazuyoshi ; Shimizu, Hiroko ; Omori, Emiko ; Morimatsu, Hiroshi ; Maeda, Shigeru ; Katayama, Hiroshi ; Nakao, Atsunori ; Morita, Kiyoshi. / Protective effect of carbon monoxide inhalation on lung injury after hemorrhagic shock/resuscitation in rats. In: Journal of Trauma - Injury, Infection and Critical Care. 2010 ; Vol. 69, No. 1. pp. 185-194.
@article{b7c69cb4bd2d47e181dd610b55eb9d9d,
title = "Protective effect of carbon monoxide inhalation on lung injury after hemorrhagic shock/resuscitation in rats",
abstract = "Background: Hemorrhagic shock and resuscitation (HSR) induces pulmonary inflammation that leads to acute lung injury. Carbon monoxide (CO), a by-product of heme catalysis, was shown to have potent cytoprotective and anti-inflammatory effects. The aim of this study was to examine the effects of CO inhalation at low concentration on lung injury induced by HSR in rats. Methods: Rats were subjected to HSR by bleeding to achieve mean arterial pressure of 30 mm Hg for 60 minutes followed by resuscitation with shed blood and saline as needed to restore blood pressure. HSR animals were either maintained in room air or were exposed to CO at 250 ppm for 1 hour before and 3 hours after HSR. Results: HSR caused an increase in the DNA binding activity of nuclear factor-κB and activator protein-1 in the lung followed by the up-regulation of pulmonary gene expression of tumor necrosis factor-α, inducible nitric oxide synthase, and interleukin (IL)-10. HSR also resulted in an increase in myeloperoxidase activity and wet weight to dry weight ratio in the lung, and more prominent histopathologic changes including congestion, edema, cellular infiltration, and hemorrhage. In contrast, CO inhalation significantly ameliorated these inflammatory events as judged by fewer histologic changes, less up-regulation of inflammatory mediators, and less activation of nuclear factor-κB and activator protein-1. Interestingly, the protective effects against lung injury afforded by CO were associated with further increases in mRNA expression of IL-10 in the lung. Conclusions: These findings suggest that inhaled CO at a low concentration ameliorated HSR-induced lung injury and attenuated inflammatory cascades by up-regulation of anti-inflammatory IL-10.",
keywords = "Activator protein-1, Acute lung injury, Carbon monoxide, Inflammation, Interleukin-10, Nuclear factor-κB",
author = "Fumitoshi Kanagawa and Toru Takahashi and Kazuyoshi Inoue and Hiroko Shimizu and Emiko Omori and Hiroshi Morimatsu and Shigeru Maeda and Hiroshi Katayama and Atsunori Nakao and Kiyoshi Morita",
year = "2010",
month = "7",
doi = "10.1097/TA.0b013e3181bbd516",
language = "English",
volume = "69",
pages = "185--194",
journal = "Journal of Trauma and Acute Care Surgery",
issn = "2163-0755",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Protective effect of carbon monoxide inhalation on lung injury after hemorrhagic shock/resuscitation in rats

AU - Kanagawa, Fumitoshi

AU - Takahashi, Toru

AU - Inoue, Kazuyoshi

AU - Shimizu, Hiroko

AU - Omori, Emiko

AU - Morimatsu, Hiroshi

AU - Maeda, Shigeru

AU - Katayama, Hiroshi

AU - Nakao, Atsunori

AU - Morita, Kiyoshi

PY - 2010/7

Y1 - 2010/7

N2 - Background: Hemorrhagic shock and resuscitation (HSR) induces pulmonary inflammation that leads to acute lung injury. Carbon monoxide (CO), a by-product of heme catalysis, was shown to have potent cytoprotective and anti-inflammatory effects. The aim of this study was to examine the effects of CO inhalation at low concentration on lung injury induced by HSR in rats. Methods: Rats were subjected to HSR by bleeding to achieve mean arterial pressure of 30 mm Hg for 60 minutes followed by resuscitation with shed blood and saline as needed to restore blood pressure. HSR animals were either maintained in room air or were exposed to CO at 250 ppm for 1 hour before and 3 hours after HSR. Results: HSR caused an increase in the DNA binding activity of nuclear factor-κB and activator protein-1 in the lung followed by the up-regulation of pulmonary gene expression of tumor necrosis factor-α, inducible nitric oxide synthase, and interleukin (IL)-10. HSR also resulted in an increase in myeloperoxidase activity and wet weight to dry weight ratio in the lung, and more prominent histopathologic changes including congestion, edema, cellular infiltration, and hemorrhage. In contrast, CO inhalation significantly ameliorated these inflammatory events as judged by fewer histologic changes, less up-regulation of inflammatory mediators, and less activation of nuclear factor-κB and activator protein-1. Interestingly, the protective effects against lung injury afforded by CO were associated with further increases in mRNA expression of IL-10 in the lung. Conclusions: These findings suggest that inhaled CO at a low concentration ameliorated HSR-induced lung injury and attenuated inflammatory cascades by up-regulation of anti-inflammatory IL-10.

AB - Background: Hemorrhagic shock and resuscitation (HSR) induces pulmonary inflammation that leads to acute lung injury. Carbon monoxide (CO), a by-product of heme catalysis, was shown to have potent cytoprotective and anti-inflammatory effects. The aim of this study was to examine the effects of CO inhalation at low concentration on lung injury induced by HSR in rats. Methods: Rats were subjected to HSR by bleeding to achieve mean arterial pressure of 30 mm Hg for 60 minutes followed by resuscitation with shed blood and saline as needed to restore blood pressure. HSR animals were either maintained in room air or were exposed to CO at 250 ppm for 1 hour before and 3 hours after HSR. Results: HSR caused an increase in the DNA binding activity of nuclear factor-κB and activator protein-1 in the lung followed by the up-regulation of pulmonary gene expression of tumor necrosis factor-α, inducible nitric oxide synthase, and interleukin (IL)-10. HSR also resulted in an increase in myeloperoxidase activity and wet weight to dry weight ratio in the lung, and more prominent histopathologic changes including congestion, edema, cellular infiltration, and hemorrhage. In contrast, CO inhalation significantly ameliorated these inflammatory events as judged by fewer histologic changes, less up-regulation of inflammatory mediators, and less activation of nuclear factor-κB and activator protein-1. Interestingly, the protective effects against lung injury afforded by CO were associated with further increases in mRNA expression of IL-10 in the lung. Conclusions: These findings suggest that inhaled CO at a low concentration ameliorated HSR-induced lung injury and attenuated inflammatory cascades by up-regulation of anti-inflammatory IL-10.

KW - Activator protein-1

KW - Acute lung injury

KW - Carbon monoxide

KW - Inflammation

KW - Interleukin-10

KW - Nuclear factor-κB

UR - http://www.scopus.com/inward/record.url?scp=77954747213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954747213&partnerID=8YFLogxK

U2 - 10.1097/TA.0b013e3181bbd516

DO - 10.1097/TA.0b013e3181bbd516

M3 - Article

VL - 69

SP - 185

EP - 194

JO - Journal of Trauma and Acute Care Surgery

JF - Journal of Trauma and Acute Care Surgery

SN - 2163-0755

IS - 1

ER -