Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats

Takehit Suzuki; Hideo Fukuoka; Setsuo Ushikoshi; Reiichiro Sato; Hidetoshi Morita; Tatsuya Takizawa

doi:10.1111/asj.12328

Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats

Takehit Suzuki, Hideo Fukuoka, Setsuo Ushikoshi, Reiichiro Sato, Hidetoshi Morita, Tatsuya Takizawa

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl₄)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl₄ (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl₄. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2′-deoxyuridine incorporation at 48h after CCl₄ treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl₄ administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl₄ treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl₄ administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.

Original language	English
Pages (from-to)	532-540
Number of pages	9
Journal	Animal Science Journal
Volume	86
Issue number	5
DOIs	https://doi.org/10.1111/asj.12328
Publication status	Published - May 1 2015

Keywords

Insulin-like growth factor 1 (IGF-1)
Liver injury
Rhizopus oryzae

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)

Access to Document

10.1111/asj.12328

Fingerprint Dive into the research topics of 'Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats'. Together they form a unique fingerprint.

Cite this

@article{fae5f221311c4dc88f8ba7175dea8ee4,

title = "Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats",

abstract = "Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl4)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl4 (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl4. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2′-deoxyuridine incorporation at 48h after CCl4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl4 treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl4 administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.",

keywords = "Insulin-like growth factor 1 (IGF-1), Liver injury, Rhizopus oryzae",

author = "Takehit Suzuki and Hideo Fukuoka and Setsuo Ushikoshi and Reiichiro Sato and Hidetoshi Morita and Tatsuya Takizawa",

year = "2015",

month = may,

day = "1",

doi = "10.1111/asj.12328",

language = "English",

volume = "86",

pages = "532--540",

journal = "Animal Science Journal",

issn = "1344-3941",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats

AU - Suzuki, Takehit

AU - Fukuoka, Hideo

AU - Ushikoshi, Setsuo

AU - Sato, Reiichiro

AU - Morita, Hidetoshi

AU - Takizawa, Tatsuya

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl4)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl4 (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl4. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2′-deoxyuridine incorporation at 48h after CCl4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl4 treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl4 administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.

AB - Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl4)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl4 (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl4. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2′-deoxyuridine incorporation at 48h after CCl4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl4 treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl4 administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.

KW - Insulin-like growth factor 1 (IGF-1)

KW - Liver injury

KW - Rhizopus oryzae

UR - http://www.scopus.com/inward/record.url?scp=84928421577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928421577&partnerID=8YFLogxK

U2 - 10.1111/asj.12328

DO - 10.1111/asj.12328

M3 - Article

C2 - 25496319

AN - SCOPUS:84928421577

VL - 86

SP - 532

EP - 540

JO - Animal Science Journal

JF - Animal Science Journal

SN - 1344-3941

IS - 5

ER -