Protection against ischemic brain damage by GDNF affecting cell survival and death signals

G. Jin, N. Omori, F. Li, I. Nagano, Y. Manabe, M. Shoji, K. Abe

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Neuroprotective effects of glial cell line-derived neurotrophic factor (GDNF) on cell survival and death signals were investigated after 90min of transient middle cerebral artery occlusion (MCAO) in rats. Immunoreactivities of phosphorylated Akt (p-Akt), cleaved caspase-9 (c-cas9), and -3 (c-cas3) increased after the reperfusion in the penumbra in vehicle group with peaks at 3 h, 8 h, and 1 day, respectively. Topical application of GDNF (6.8 μg/9 μl) on brain surface potentiated and prolonged-Akt activation, but suppressed activation of the caspases, and reduced the number of terminal deoxynucleotidyl transferasemediated dUDP-biotin in situ nick labeling (TUNEL) positive cells. These results suggest that GDNF plays a protective role against ischemic injury by controlling the balance between Akt pathway and caspase cascades.

Original languageEnglish
Pages (from-to)249-253
Number of pages5
JournalNeurological Research
Volume25
Issue number3
DOIs
Publication statusPublished - Apr 1 2003

Keywords

  • Akt
  • Caspase-3
  • Caspase-9
  • Glial cell line-derived neurotrophic factor (GDNF)
  • Ischemia
  • Rat

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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