Neuroprotective effects of glial cell line-derived neurotrophic factor (GDNF) on cell survival and death signals were investigated after 90min of transient middle cerebral artery occlusion (MCAO) in rats. Immunoreactivities of phosphorylated Akt (p-Akt), cleaved caspase-9 (c-cas9), and -3 (c-cas3) increased after the reperfusion in the penumbra in vehicle group with peaks at 3 h, 8 h, and 1 day, respectively. Topical application of GDNF (6.8 μg/9 μl) on brain surface potentiated and prolonged-Akt activation, but suppressed activation of the caspases, and reduced the number of terminal deoxynucleotidyl transferasemediated dUDP-biotin in situ nick labeling (TUNEL) positive cells. These results suggest that GDNF plays a protective role against ischemic injury by controlling the balance between Akt pathway and caspase cascades.
- Glial cell line-derived neurotrophic factor (GDNF)
ASJC Scopus subject areas
- Clinical Neurology