Proteases augment the effects of lipopolysaccharide in rat gingiva

Daisuke Ekuni, T. Yamamoto, Reiko Yamanaka, K. Tachibana, T. Watanabe

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objectives: Lipopolysaccharide (LPS) and proteases have been implicated as important factors in the initiation and progression of human periodontal diseases. A single application of LPS or proteases is insufficient to induce periodontal pocket formation or periodontitis. The aim of the present study was to assess the combined effect of lipopolysaccharide and proteases on rat periodontal tissues, and create a periodontal disease model. Materials and methods: Forty male Wistar rats were divided into four groups: combination group (treated with both LPS and proteases solutions); LPS group; proteases group; and control. Each solution was introduced daily into the palatal gingival sulcus of maxillary molars for 8 weeks. The tissues were evaluated histometrically and immunohistochemically. Results: In the LPS group, elongation of rete ridge, apical migration of junctional epithelium (JE), increased numbers of B cells in connective tissue, and resorption of alveolar bone were observed. In the proteases group, the increase in the number of infiltrating polymorphonuclear leukocytes and blood vessels in the connective tissue was greater than that of the LPS group. Conclusions: The effects of LPS on periodontal tissues differed from those of proteases. The addition of proteases augmented and increased the effects of LPS, which were apical migration, intraepithelial cleavage of JE, and increased B cell density. The lesions in the combination group resembled established lesions of human periodontitis, with the exception of the low density of plasma cells.

Original languageEnglish
Pages (from-to)591-596
Number of pages6
JournalJournal of Periodontal Research
Volume38
Issue number6
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Gingiva
Lipopolysaccharides
Peptide Hydrolases
Epithelial Attachment
Periodontitis
Periodontal Diseases
Connective Tissue
B-Lymphocytes
Periodontal Pocket
Alveolar Bone Loss
Peptide Initiation Factors
Bone Resorption
Plasma Cells
Blood Vessels
Wistar Rats
Neutrophils
Cell Count

Keywords

  • Additional effect
  • Lipopolysaccharides
  • Periodontal diseases
  • Proteases

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Proteases augment the effects of lipopolysaccharide in rat gingiva. / Ekuni, Daisuke; Yamamoto, T.; Yamanaka, Reiko; Tachibana, K.; Watanabe, T.

In: Journal of Periodontal Research, Vol. 38, No. 6, 12.2003, p. 591-596.

Research output: Contribution to journalArticle

Ekuni, Daisuke ; Yamamoto, T. ; Yamanaka, Reiko ; Tachibana, K. ; Watanabe, T. / Proteases augment the effects of lipopolysaccharide in rat gingiva. In: Journal of Periodontal Research. 2003 ; Vol. 38, No. 6. pp. 591-596.
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