Protease susceptibility of β-lactoglobulin adsorbed on stainless steel surface as evidence of contribution of its specific segment to adsorption

Takaharu Sakiyama, Atsuko Aya, Mari Embutsu, Koreyoshi Imamura, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

β-Lactoglobulin (β-Lg) is a major constituent of fouling deposits in the dairy industry. To determine the interaction between β-Lg and stainless steel surfaces, β-Lg irreversibly adsorbed on stainless steel particles was subjected to lysyl endopeptidase treatment and the course of fragmentation was compared with that observed for β-Lg in solution. The results showed a distinct difference between the courses of fragmentation: a fragment (residues 102-135) was liberated readily from β-Lg in solution but scarcely from β-Lg irreversibly adsorbed on stainless steel particles. This result strongly suggests that residues 102-135 include a segment primarily responsible for the interaction of β-Lg with stainless steel surfaces. This supports our previous results [Sakiyama et al., J. Biosci. Bioeng., 88, 536-541 (1999)] that showed that residues 125-135 of β-Lg have a strong affinity toward stainless steel surfaces and probably a major contribution to the adsorption of β-Lg.

Original languageEnglish
Pages (from-to)434-439
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume101
Issue number5
DOIs
Publication statusPublished - May 2006

Fingerprint

Lactoglobulins
Stainless Steel
Adsorption
Peptide Hydrolases
Stainless steel
lysyl endopeptidase
Dairying
Dairies
Fouling
Deposits
Industry

Keywords

  • β-lactoglobulin
  • lysyl endopeptidase
  • peptide fragment
  • stainless steel surface

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Protease susceptibility of β-lactoglobulin adsorbed on stainless steel surface as evidence of contribution of its specific segment to adsorption. / Sakiyama, Takaharu; Aya, Atsuko; Embutsu, Mari; Imamura, Koreyoshi; Nakanishi, Kazuhiro.

In: Journal of Bioscience and Bioengineering, Vol. 101, No. 5, 05.2006, p. 434-439.

Research output: Contribution to journalArticle

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