Protamine augments stretch induced calcium increase in vascular endothelium

Kichiro Murase, Keiji Naruse, Akira Kimura, Kenji Okumura, Tetsuo Hayakawa, Masahiro Sokabe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Human umbilical vein endothelial cells cultured on a transparent silicone chamber were subjected to a short stretch pulse (ca. 1 s, 5 - 25% stretch) of their substrate and following increases in intracellular Ca 2+ concentration ([Ca 2+] i) were measured by fluorescence intensity ratiometry using fura-2. In response to mechanical stretch, the cells in HEPES buffered saline exhibited a Ca 2+ transient in a dose dependent way. The response was completely dependent on external Ca 2+ and inhibited by gadolinium (Gd 3+), suggesting that ir was mediated by the activation of a stretch activated cation channel (SACatC). Interestingly, the stretch induced Ca 2+ transient was significantly augmented in the presence of basic polypeptide, protamine. This augmented Ca 2+ response was inhibited neither by Gd 3+ nor by the deprivation of external Ca 2+, indicating that the SACatC is not responsible for this phenomenon. In contrast, this augmentation was inhibited by depletion of intracellular Ca 2+ stores with thapsigargin or by the pretreatment with phospholipase inhibitors such as U73122 and manoalide. 5 These results suggest the presence of a metabotropic mechanoreceptor distinct from the SACatC in vascular endothelium. This augmented [Ca 2-] i increase may contribute to the vasodilating response induced by protamine during heparin neutralization in cardiac surgery.

Original languageEnglish
Pages (from-to)1403-1410
Number of pages8
JournalBritish Journal of Pharmacology
Volume134
Issue number7
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Protamines
Vascular Endothelium
Cations
Calcium
HEPES
Mechanoreceptors
Thapsigargin
Phospholipases
Fura-2
Human Umbilical Vein Endothelial Cells
Gadolinium
Silicones
Thoracic Surgery
Pulse
Heparin
Fluorescence
Peptides

Keywords

  • Calcium
  • Mechanoreceptor
  • Protamine
  • Stretch activated cation channel
  • Vascular endothelium

ASJC Scopus subject areas

  • Pharmacology

Cite this

Murase, K., Naruse, K., Kimura, A., Okumura, K., Hayakawa, T., & Sokabe, M. (2001). Protamine augments stretch induced calcium increase in vascular endothelium. British Journal of Pharmacology, 134(7), 1403-1410.

Protamine augments stretch induced calcium increase in vascular endothelium. / Murase, Kichiro; Naruse, Keiji; Kimura, Akira; Okumura, Kenji; Hayakawa, Tetsuo; Sokabe, Masahiro.

In: British Journal of Pharmacology, Vol. 134, No. 7, 2001, p. 1403-1410.

Research output: Contribution to journalArticle

Murase, K, Naruse, K, Kimura, A, Okumura, K, Hayakawa, T & Sokabe, M 2001, 'Protamine augments stretch induced calcium increase in vascular endothelium', British Journal of Pharmacology, vol. 134, no. 7, pp. 1403-1410.
Murase K, Naruse K, Kimura A, Okumura K, Hayakawa T, Sokabe M. Protamine augments stretch induced calcium increase in vascular endothelium. British Journal of Pharmacology. 2001;134(7):1403-1410.
Murase, Kichiro ; Naruse, Keiji ; Kimura, Akira ; Okumura, Kenji ; Hayakawa, Tetsuo ; Sokabe, Masahiro. / Protamine augments stretch induced calcium increase in vascular endothelium. In: British Journal of Pharmacology. 2001 ; Vol. 134, No. 7. pp. 1403-1410.
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