Prostaglandin I1 analogues, SM-10902 and SM-10906, affect human keratinocytes and fibroblasts in vitro in a manner similar to PGE1: therapeutic potential for wound healing

F. Kaneko, J. Z. Zhang, K. Maruyama, Y. Nihei, I. Ono, K. Iwatsuki, T. Yamamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The newly synthesized prostaglandin (PG) I1 analogues, SM-10902 and SM-10906, were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) in order to evaluate their therapeutic potential for cutaneous wound healing. The PGI1 analogues had a direct effect on cell proliferation of HDFs as did PGE1, but inibited cell growth of NHKs in contrast to the stimulatory effect observed with PGE1. In contrast to NHKs stimulated with PGI1 analogues, which exhibited low levels of adenosine 3,5-cyclic monophosphate (cAMP). HDFs stimulated with these analogues responded in a dose-dependent manner with extremely high levels of cAMP. Conditioned media (CM) derived from media in which HDFs had been incubated with both the PGI1 analogues promoted NHK proliferation. HDF production of interleukin (IL)-6 increased in response to the PGI1 analogues. Since IL-6 was shown to promote cell growth of NHKs, enhancement of NHK proliferation by CM was thought to be due to IL-6 derived from HDFs stimulated with the PGI1 analogues.

Original languageEnglish
Pages (from-to)539-545
Number of pages7
JournalArchives of Dermatological Research
Volume287
Issue number6
DOIs
Publication statusPublished - Aug 1995
Externally publishedYes

Fingerprint

SM 10902
Synthetic Prostaglandins
Alprostadil
Keratinocytes
Wound Healing
Fibroblasts
Skin
Therapeutics
Interleukin-6
Conditioned Culture Medium
In Vitro Techniques
SM 10906

Keywords

  • Dermal fibroblasts
  • Keratinocytes
  • Prostaglandin E
  • Prostaglandin I analogues
  • Wound healing

ASJC Scopus subject areas

  • Dermatology

Cite this

Prostaglandin I1 analogues, SM-10902 and SM-10906, affect human keratinocytes and fibroblasts in vitro in a manner similar to PGE1 : therapeutic potential for wound healing. / Kaneko, F.; Zhang, J. Z.; Maruyama, K.; Nihei, Y.; Ono, I.; Iwatsuki, K.; Yamamoto, T.

In: Archives of Dermatological Research, Vol. 287, No. 6, 08.1995, p. 539-545.

Research output: Contribution to journalArticle

Kaneko, F. ; Zhang, J. Z. ; Maruyama, K. ; Nihei, Y. ; Ono, I. ; Iwatsuki, K. ; Yamamoto, T. / Prostaglandin I1 analogues, SM-10902 and SM-10906, affect human keratinocytes and fibroblasts in vitro in a manner similar to PGE1 : therapeutic potential for wound healing. In: Archives of Dermatological Research. 1995 ; Vol. 287, No. 6. pp. 539-545.
@article{72e03eb5b73f47d296bea96b40c6cbb8,
title = "Prostaglandin I1 analogues, SM-10902 and SM-10906, affect human keratinocytes and fibroblasts in vitro in a manner similar to PGE1: therapeutic potential for wound healing",
abstract = "The newly synthesized prostaglandin (PG) I1 analogues, SM-10902 and SM-10906, were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) in order to evaluate their therapeutic potential for cutaneous wound healing. The PGI1 analogues had a direct effect on cell proliferation of HDFs as did PGE1, but inibited cell growth of NHKs in contrast to the stimulatory effect observed with PGE1. In contrast to NHKs stimulated with PGI1 analogues, which exhibited low levels of adenosine 3,5-cyclic monophosphate (cAMP). HDFs stimulated with these analogues responded in a dose-dependent manner with extremely high levels of cAMP. Conditioned media (CM) derived from media in which HDFs had been incubated with both the PGI1 analogues promoted NHK proliferation. HDF production of interleukin (IL)-6 increased in response to the PGI1 analogues. Since IL-6 was shown to promote cell growth of NHKs, enhancement of NHK proliferation by CM was thought to be due to IL-6 derived from HDFs stimulated with the PGI1 analogues.",
keywords = "Dermal fibroblasts, Keratinocytes, Prostaglandin E, Prostaglandin I analogues, Wound healing",
author = "F. Kaneko and Zhang, {J. Z.} and K. Maruyama and Y. Nihei and I. Ono and K. Iwatsuki and T. Yamamoto",
year = "1995",
month = "8",
doi = "10.1007/BF00374073",
language = "English",
volume = "287",
pages = "539--545",
journal = "Archives of Dermatological Research",
issn = "0340-3696",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Prostaglandin I1 analogues, SM-10902 and SM-10906, affect human keratinocytes and fibroblasts in vitro in a manner similar to PGE1

T2 - therapeutic potential for wound healing

AU - Kaneko, F.

AU - Zhang, J. Z.

AU - Maruyama, K.

AU - Nihei, Y.

AU - Ono, I.

AU - Iwatsuki, K.

AU - Yamamoto, T.

PY - 1995/8

Y1 - 1995/8

N2 - The newly synthesized prostaglandin (PG) I1 analogues, SM-10902 and SM-10906, were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) in order to evaluate their therapeutic potential for cutaneous wound healing. The PGI1 analogues had a direct effect on cell proliferation of HDFs as did PGE1, but inibited cell growth of NHKs in contrast to the stimulatory effect observed with PGE1. In contrast to NHKs stimulated with PGI1 analogues, which exhibited low levels of adenosine 3,5-cyclic monophosphate (cAMP). HDFs stimulated with these analogues responded in a dose-dependent manner with extremely high levels of cAMP. Conditioned media (CM) derived from media in which HDFs had been incubated with both the PGI1 analogues promoted NHK proliferation. HDF production of interleukin (IL)-6 increased in response to the PGI1 analogues. Since IL-6 was shown to promote cell growth of NHKs, enhancement of NHK proliferation by CM was thought to be due to IL-6 derived from HDFs stimulated with the PGI1 analogues.

AB - The newly synthesized prostaglandin (PG) I1 analogues, SM-10902 and SM-10906, were compared with PGE1 in terms of their biological effects on cultured normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) in order to evaluate their therapeutic potential for cutaneous wound healing. The PGI1 analogues had a direct effect on cell proliferation of HDFs as did PGE1, but inibited cell growth of NHKs in contrast to the stimulatory effect observed with PGE1. In contrast to NHKs stimulated with PGI1 analogues, which exhibited low levels of adenosine 3,5-cyclic monophosphate (cAMP). HDFs stimulated with these analogues responded in a dose-dependent manner with extremely high levels of cAMP. Conditioned media (CM) derived from media in which HDFs had been incubated with both the PGI1 analogues promoted NHK proliferation. HDF production of interleukin (IL)-6 increased in response to the PGI1 analogues. Since IL-6 was shown to promote cell growth of NHKs, enhancement of NHK proliferation by CM was thought to be due to IL-6 derived from HDFs stimulated with the PGI1 analogues.

KW - Dermal fibroblasts

KW - Keratinocytes

KW - Prostaglandin E

KW - Prostaglandin I analogues

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=0028990695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028990695&partnerID=8YFLogxK

U2 - 10.1007/BF00374073

DO - 10.1007/BF00374073

M3 - Article

C2 - 7487139

AN - SCOPUS:0028990695

VL - 287

SP - 539

EP - 545

JO - Archives of Dermatological Research

JF - Archives of Dermatological Research

SN - 0340-3696

IS - 6

ER -