Prostaglandin F regulates the nitric oxide generating system in bovine luteal endothelial cells

Seung Hyung Lee, Tomas J. Acosta, Shin Yoshioka, Kiyoshi Okuda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The objective of the present study was to elucidate whether luteolytic prostaglandin F (PGF) plays roles in regulating the nitric oxide (NO) generating system in luteal endothelial cells (LECs). Reverse transcriptase PCR, immunoblotting and immunostaining revealed the presence of PGF receptor mRNA (521 bp) and protein (64 kDa) in cultured LECs obtained from the mid-stage corpus luteum. When cultured LECs were exposed to 0.1 μM-10 μM PGF, NO production was significantly stimulated by PGF at 24 h. When LECs were exposed to 1 μM PGF for 2, 6 and 24 h, PGF did not affect the expressions of endothelial NO synthase (eNOS) mRNA and protein. On the other hand, PGF stimulated the expression of inducible NOS (iNOS) mRNA (P<0.05) and protein (P<0.05) at 2 h, but not at 6 and 24 h. By observing the conversion of [3C]L-arginine to [3C]L-citrulline, we found that PGF stimulated NOS activity in cultured LECs at 2 h (P<0.05). The overall findings indicate that bovine LECs are a target for PGF and that PGF stimulates iNOS expression and NOS activity in bovine LECs. Stimulation of the NO generating system and NOS activity by PGF may result in increasing local NO production followed by luteolysis.

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalJournal of Reproduction and Development
Volume55
Issue number4
DOIs
Publication statusPublished - Oct 12 2009

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Keywords

  • Luteal endothelial cell
  • Nitric oxide
  • Prostaglandin F(PGF)
  • eNOS
  • iNOS

ASJC Scopus subject areas

  • Animal Science and Zoology

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