Prostaglandin F2α regulates the nitric oxide generating system in bovine luteal endothelial cells

Seung Hyung Lee, Tomas J. Acosta, Shin Yoshioka, Kiyoshi Okuda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The objective of the present study was to elucidate whether luteolytic prostaglandin F (PGF) plays roles in regulating the nitric oxide (NO) generating system in luteal endothelial cells (LECs). Reverse transcriptase PCR, immunoblotting and immunostaining revealed the presence of PGF receptor mRNA (521 bp) and protein (64 kDa) in cultured LECs obtained from the mid-stage corpus luteum. When cultured LECs were exposed to 0.1 μM-10 μM PGF, NO production was significantly stimulated by PGF at 24 h. When LECs were exposed to 1 μM PGF for 2, 6 and 24 h, PGF did not affect the expressions of endothelial NO synthase (eNOS) mRNA and protein. On the other hand, PGF stimulated the expression of inducible NOS (iNOS) mRNA (P3C]L-arginine to [3C]L-citrulline, we found that PGF stimulated NOS activity in cultured LECs at 2 h (P

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalJournal of Reproduction and Development
Volume55
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

luteal cells
prostaglandins
endothelial cells
nitric oxide
cattle
citrulline
immunoblotting
corpus luteum
arginine
proteins
reverse transcriptase polymerase chain reaction
receptors

Keywords

  • eNOS
  • iNOS
  • Luteal endothelial cell
  • Nitric oxide
  • Prostaglandin F(PGF)

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Prostaglandin F2α regulates the nitric oxide generating system in bovine luteal endothelial cells. / Lee, Seung Hyung; Acosta, Tomas J.; Yoshioka, Shin; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 55, No. 4, 2009, p. 418-424.

Research output: Contribution to journalArticle

Lee, Seung Hyung ; Acosta, Tomas J. ; Yoshioka, Shin ; Okuda, Kiyoshi. / Prostaglandin F2α regulates the nitric oxide generating system in bovine luteal endothelial cells. In: Journal of Reproduction and Development. 2009 ; Vol. 55, No. 4. pp. 418-424.
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