Prostacyclin synthase gene transfer modulates cyclooxygenase-2-derived prostanoid synthesis and inhibits neointimal formation in rat balloon-injured arteries

Michiharu Yamada, Yasushi Numaguchi, Kenji Okumura, Mitsunori Harada, Keiji Naruse, Hideo Matsui, Takayuki Ito, Tetsuo Hayakawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Previous studies have shown that prostacyclin (PGI2) synthase (PCS) gene transfer inhibits neointimal formation in balloon-injured arteries. However, the role of each cyclooxygenase (COX) isoform in this healing mechanism remains unknown. We hypothesized that overexpression of PCS may modulate COX-2-mediated prostaglandin (PG) metabolism. That is to say, excessive PGH2 derived from COX-2 after balloon injury may be converted into PGI2 rather than PGE2 or thromboxane (TX) A2 by overexpressed PCS. We examined the expression of COX isoforms and evaluated the role of COX-2 with regard to the effects of PCS gene transfer by using 4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzenesulfonamide (JTE-522), a selective COX-2 inhibitor. Rats were divided into 4 groups in conjunction with PCS gene transfer and JTE-522 treatment. The PCS gene (30 μg) was transfected into rat balloon-injured arteries by a lipotransfection method. JTE-522 (30 mg/kg per day) was administered for 14 days after balloon injury. Immunohistochemical analysis demonstrated marked COX-2 expression on the neointima. PCS gene transfer markedly inhibited neointimal formation, but JTE-522 reversed this beneficial effect. PCS gene transfer augmented PGI2 production and decreased PGE2 production without affecting TXA2 production, but JTE-522 inhibited this increase in PGI2 production. In conclusion, PCS gene transfer modulated COX-2-mediated prostanoid synthesis and inhibited neointimal formation after balloon injury.

Original languageEnglish
Pages (from-to)256-262
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Prostaglandins
Arteries
Genes
Epoprostenol
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Wounds and Injuries
Protein Isoforms
Prostaglandin H2
Neointima
Thromboxane A2
Cyclooxygenase 2 Inhibitors
prostacyclin synthetase
4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzenesulfonamide

Keywords

  • Balloon injury
  • Cyclooxygenase-2
  • Gene therapy
  • Prostacyclin
  • Restenosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Prostacyclin synthase gene transfer modulates cyclooxygenase-2-derived prostanoid synthesis and inhibits neointimal formation in rat balloon-injured arteries. / Yamada, Michiharu; Numaguchi, Yasushi; Okumura, Kenji; Harada, Mitsunori; Naruse, Keiji; Matsui, Hideo; Ito, Takayuki; Hayakawa, Tetsuo.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 22, No. 2, 2002, p. 256-262.

Research output: Contribution to journalArticle

Yamada, Michiharu ; Numaguchi, Yasushi ; Okumura, Kenji ; Harada, Mitsunori ; Naruse, Keiji ; Matsui, Hideo ; Ito, Takayuki ; Hayakawa, Tetsuo. / Prostacyclin synthase gene transfer modulates cyclooxygenase-2-derived prostanoid synthesis and inhibits neointimal formation in rat balloon-injured arteries. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2002 ; Vol. 22, No. 2. pp. 256-262.
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AU - Yamada, Michiharu

AU - Numaguchi, Yasushi

AU - Okumura, Kenji

AU - Harada, Mitsunori

AU - Naruse, Keiji

AU - Matsui, Hideo

AU - Ito, Takayuki

AU - Hayakawa, Tetsuo

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N2 - Previous studies have shown that prostacyclin (PGI2) synthase (PCS) gene transfer inhibits neointimal formation in balloon-injured arteries. However, the role of each cyclooxygenase (COX) isoform in this healing mechanism remains unknown. We hypothesized that overexpression of PCS may modulate COX-2-mediated prostaglandin (PG) metabolism. That is to say, excessive PGH2 derived from COX-2 after balloon injury may be converted into PGI2 rather than PGE2 or thromboxane (TX) A2 by overexpressed PCS. We examined the expression of COX isoforms and evaluated the role of COX-2 with regard to the effects of PCS gene transfer by using 4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzenesulfonamide (JTE-522), a selective COX-2 inhibitor. Rats were divided into 4 groups in conjunction with PCS gene transfer and JTE-522 treatment. The PCS gene (30 μg) was transfected into rat balloon-injured arteries by a lipotransfection method. JTE-522 (30 mg/kg per day) was administered for 14 days after balloon injury. Immunohistochemical analysis demonstrated marked COX-2 expression on the neointima. PCS gene transfer markedly inhibited neointimal formation, but JTE-522 reversed this beneficial effect. PCS gene transfer augmented PGI2 production and decreased PGE2 production without affecting TXA2 production, but JTE-522 inhibited this increase in PGI2 production. In conclusion, PCS gene transfer modulated COX-2-mediated prostanoid synthesis and inhibited neointimal formation after balloon injury.

AB - Previous studies have shown that prostacyclin (PGI2) synthase (PCS) gene transfer inhibits neointimal formation in balloon-injured arteries. However, the role of each cyclooxygenase (COX) isoform in this healing mechanism remains unknown. We hypothesized that overexpression of PCS may modulate COX-2-mediated prostaglandin (PG) metabolism. That is to say, excessive PGH2 derived from COX-2 after balloon injury may be converted into PGI2 rather than PGE2 or thromboxane (TX) A2 by overexpressed PCS. We examined the expression of COX isoforms and evaluated the role of COX-2 with regard to the effects of PCS gene transfer by using 4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzenesulfonamide (JTE-522), a selective COX-2 inhibitor. Rats were divided into 4 groups in conjunction with PCS gene transfer and JTE-522 treatment. The PCS gene (30 μg) was transfected into rat balloon-injured arteries by a lipotransfection method. JTE-522 (30 mg/kg per day) was administered for 14 days after balloon injury. Immunohistochemical analysis demonstrated marked COX-2 expression on the neointima. PCS gene transfer markedly inhibited neointimal formation, but JTE-522 reversed this beneficial effect. PCS gene transfer augmented PGI2 production and decreased PGE2 production without affecting TXA2 production, but JTE-522 inhibited this increase in PGI2 production. In conclusion, PCS gene transfer modulated COX-2-mediated prostanoid synthesis and inhibited neointimal formation after balloon injury.

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