We examined the effect of propofol and thiopental, intravenous anesthetics, on the hyperpolarization-activated inward current (IH), whose functional role on the neuronal activity has been evaluated. Whole-cell recordings of IH evoked by hyperpolarizing step pulses were taken from hippocampal CA1 neurons in rat brain slices. Propofol reduced IH current in a dose-dependent manner. However, thiopental had no significant effect on the activation of IH. According to the functional role of IH, the suppression of IH should result in a reduction of neuronal activity. We suggest that the effectiveness of propofol as an anticonvulsant or an antiemetic is associated with the blockade of the IH channel.
- Brain slices
- Hyperpolarization-activated inward current
- Whole-cell patch-clamp
ASJC Scopus subject areas