Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1

Teruo Kuroda, Toshi Shimamoto, Kei Inaba, Takashi Kayahara, Masaaki Tsuda, Tomofusa Tsuchiya

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Abstract

The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent ntiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K++H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the Km value of the Na+/H+ antiport, but it did increase the Vmax.

Original languageEnglish
Pages (from-to)1162-1165
Number of pages4
JournalJournal of biochemistry
Volume115
Issue number6
DOIs
Publication statusPublished - Jun 1994

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Keywords

  • PH-dependence
  • Properties
  • Substrate specificity
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Kuroda, T., Shimamoto, T., Inaba, K., Kayahara, T., Tsuda, M., & Tsuchiya, T. (1994). Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1. Journal of biochemistry, 115(6), 1162-1165. https://doi.org/10.1093/oxfordjournals.jbchem.a124473