Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1

Teruo Kuroda, Toshi Shimamoto, Kei Inaba, Takashi Kayahara, Masaaki Tsuda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent ntiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K++H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the Km value of the Na+/H+ antiport, but it did increase the Vmax.

Original languageEnglish
Pages (from-to)1162-1165
Number of pages4
JournalJournal of Biochemistry
Volume115
Issue number6
Publication statusPublished - Jun 1994

Fingerprint

Antiporters
Vibrio
Sodium-Hydrogen Antiporter
Substrate
Vesicles
Ion Transport
Vibrio parahaemolyticus
Substrates
Membrane
Membranes
Dependent
Amiloride
Bacteria
Escherichia coli
Escherichia Coli
Assays

Keywords

  • ATPNa+/H+ antiporter
  • PH-dependence
  • Properties
  • Substrate specificity
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Kuroda, T., Shimamoto, T., Inaba, K., Kayahara, T., Tsuda, M., & Tsuchiya, T. (1994). Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1. Journal of Biochemistry, 115(6), 1162-1165.

Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1. / Kuroda, Teruo; Shimamoto, Toshi; Inaba, Kei; Kayahara, Takashi; Tsuda, Masaaki; Tsuchiya, Tomofusa.

In: Journal of Biochemistry, Vol. 115, No. 6, 06.1994, p. 1162-1165.

Research output: Contribution to journalArticle

Kuroda, T, Shimamoto, T, Inaba, K, Kayahara, T, Tsuda, M & Tsuchiya, T 1994, 'Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1', Journal of Biochemistry, vol. 115, no. 6, pp. 1162-1165.
Kuroda T, Shimamoto T, Inaba K, Kayahara T, Tsuda M, Tsuchiya T. Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1. Journal of Biochemistry. 1994 Jun;115(6):1162-1165.
Kuroda, Teruo ; Shimamoto, Toshi ; Inaba, Kei ; Kayahara, Takashi ; Tsuda, Masaaki ; Tsuchiya, Tomofusa. / Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1. In: Journal of Biochemistry. 1994 ; Vol. 115, No. 6. pp. 1162-1165.
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