Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)

K. Ochiai, H. Oda, S. Shono, Y. Kato, S. Sugihara, S. Nakazawa, D. Azakami, M. Michishita, E. Onozawa, M. Bonkobara, T. Sako, L. Shun-Ai, H. Ueki, M. Watanabe, T. Omi

Research output: Contribution to journalArticle

Abstract

Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.

Original languageEnglish
Pages (from-to)1181-1186
Number of pages6
JournalVeterinary and Comparative Oncology
Volume15
Issue number4
DOIs
Publication statusPublished - Dec 2017

Keywords

  • REIC/Dkk-3
  • cat
  • feline
  • mammary gland tumours
  • tumour suppressor gene

ASJC Scopus subject areas

  • veterinary(all)

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    Ochiai, K., Oda, H., Shono, S., Kato, Y., Sugihara, S., Nakazawa, S., Azakami, D., Michishita, M., Onozawa, E., Bonkobara, M., Sako, T., Shun-Ai, L., Ueki, H., Watanabe, M., & Omi, T. (2017). Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3). Veterinary and Comparative Oncology, 15(4), 1181-1186. https://doi.org/10.1111/vco.12254