Properties of hemolysin and protease produced by Aeromonas trota

Eizo Takahashi, Haruka Ozaki, Yoshio Fujii, Hidetomo Kobayashi, Hiroyasu Yamanaka, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined the properties of exotoxins produced by Aeromonas trota (A. enteropelogenes), one of the diarrheagenic species of Aeromonadaceae. Nine of 19 A. trota isolates that grew on solid media containing erythrocytes showed hemolytic activity. However, the hemolytic activities of the culture supernatants of these hemolytic strains of A. trota were markedly lower than those of A. sobria when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum against the hemolysin produced by A. sobria was also low. A mouse intestine loop assay using living bacterial cells showed that A. trota 701 caused the significant accumulation of fluid, and antiserum against the hemolysin produced suppressed the enterotoxic action of A. trota 701. These results indicated that A. trota 701 was diarrheagenic and the hemolysin produced was the causative agent of the enterotoxic activity of A. trota. The hemolysin in A. sobria was previously shown to be secreted in a preform (inactive form) and be activated when the carboxy-terminal domain was cleaved off by proteases in the culture supernatant. Since mature hemolysin was detected in the culture supernatants of A. trota, we analyzed the extracellular protease produced by A. trota. Fifteen of 19 A. trota isolates that grew on solid media containing skim milk showed proteolytic activity. We subsequently found that most A. trota isolates possessed the serine protease gene, but not the metalloprotease gene. Therefore, we determined the nucleotide sequence of the serine protease gene and its chaperone A. trota gene. The results obtained revealed that the deduced amino acid sequences of serine protease and the chaperone were homologous to those of A. sobria with identities of 83.0% and 75.8%, respectively.

Original languageEnglish
Article numbere91149
JournalPLoS One
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 14 2014

Fingerprint

Aeromonas trota
Aeromonas
Hemolysin Proteins
hemolysins
Peptide Hydrolases
proteinases
Serine Proteases
Genes
Aeromonadaceae
Immune Sera
serine proteinases
Exotoxins
Metalloproteases
Immunoblotting
Aeromonas enteropelogenes
Intestines
antiserum
Amino Acid Sequence
Assays
Milk

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Properties of hemolysin and protease produced by Aeromonas trota. / Takahashi, Eizo; Ozaki, Haruka; Fujii, Yoshio; Kobayashi, Hidetomo; Yamanaka, Hiroyasu; Arimoto, Sakae; Negishi, Tomoe; Okamoto, Keinosuke.

In: PLoS One, Vol. 9, No. 3, e91149, 14.03.2014.

Research output: Contribution to journalArticle

Takahashi, Eizo ; Ozaki, Haruka ; Fujii, Yoshio ; Kobayashi, Hidetomo ; Yamanaka, Hiroyasu ; Arimoto, Sakae ; Negishi, Tomoe ; Okamoto, Keinosuke. / Properties of hemolysin and protease produced by Aeromonas trota. In: PLoS One. 2014 ; Vol. 9, No. 3.
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