Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus

T. Honda, Y. Ni, A. Hata, M. Yoh, T. Miwatani, Keinosuke Okamoto, K. Goshima, H. Takakura, S. Tsunasawa, F. Sakiyama

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A hemolytic toxin (Vp-TRH) produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus was further characterized. The purified Vp-TRH showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (Vp-TDH), a phatogenic toxin produced by Kanagawa phenomenon positive V. parahaemolyticus. Immunological similarities of Vp-TRH not only to Vp-TDH but also to hemolytic toxins produced by Vibrio hollisae and Vibrio cholerae non-O1, both of which are also enteropathogens closely related to V. parahaemolyticus, were demonstrated. The amino acid composition and sequence of N-terminal amino acids of Vp-TRH were determined. These results suggest that Vp-TRH has biological and immunological characters similar to Vp-DTH, although they are distinct molecules.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalCanadian Journal of Microbiology
Volume36
Issue number6
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Hemolysin Proteins
Amino Acids
Vibrio cholerae non-O1
Bioactivity
Rabbits
Skin
Vibrio
Capillary Permeability
Molecules
Fluids
Amino Acid Sequence
Cultured Cells
Chemical analysis
thermostable direct hemolysin

Keywords

  • Kanagawa phenomenon
  • thermostable direct hemolysin
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus. / Honda, T.; Ni, Y.; Hata, A.; Yoh, M.; Miwatani, T.; Okamoto, Keinosuke; Goshima, K.; Takakura, H.; Tsunasawa, S.; Sakiyama, F.

In: Canadian Journal of Microbiology, Vol. 36, No. 6, 1990, p. 395-399.

Research output: Contribution to journalArticle

Honda, T, Ni, Y, Hata, A, Yoh, M, Miwatani, T, Okamoto, K, Goshima, K, Takakura, H, Tsunasawa, S & Sakiyama, F 1990, 'Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus', Canadian Journal of Microbiology, vol. 36, no. 6, pp. 395-399.
Honda, T. ; Ni, Y. ; Hata, A. ; Yoh, M. ; Miwatani, T. ; Okamoto, Keinosuke ; Goshima, K. ; Takakura, H. ; Tsunasawa, S. ; Sakiyama, F. / Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus. In: Canadian Journal of Microbiology. 1990 ; Vol. 36, No. 6. pp. 395-399.
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