Abstract
We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrBKp gene and a part of the acrAKp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrAKpBKp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrAKp BKp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAKKpBKp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrAKp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.
| Original language | English |
|---|---|
| Pages (from-to) | 577-582 |
| Number of pages | 6 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2008 |
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Keywords
- AcrAB-KocC
- Drug resistance
- Klebsiella pneumoniae
- Multidrug efflux pump
- Resistance nodulation cell-division
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Properties and expression of a multidrug efflux pump AcrAB-KocC from Klebsiella pneumoniae. / Li, Dai Wei; Onishi, Motoyasu; Kishino, Takanori; Matsuo, Taira; Ogawa, Wakano; Kuroda, Teruo; Tsuchiya, Tomofusa.
In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 4, 04.2008, p. 577-582.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Properties and expression of a multidrug efflux pump AcrAB-KocC from Klebsiella pneumoniae
AU - Li, Dai Wei
AU - Onishi, Motoyasu
AU - Kishino, Takanori
AU - Matsuo, Taira
AU - Ogawa, Wakano
AU - Kuroda, Teruo
AU - Tsuchiya, Tomofusa
PY - 2008/4
Y1 - 2008/4
N2 - We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrBKp gene and a part of the acrAKp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrAKpBKp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrAKp BKp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAKKpBKp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrAKp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.
AB - We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrBKp gene and a part of the acrAKp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrAKpBKp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrAKp BKp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAKKpBKp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrAKp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.
KW - AcrAB-KocC
KW - Drug resistance
KW - Klebsiella pneumoniae
KW - Multidrug efflux pump
KW - Resistance nodulation cell-division
UR - http://www.scopus.com/inward/record.url?scp=41849149702&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41849149702&partnerID=8YFLogxK
U2 - 10.1248/bpb.31.577
DO - 10.1248/bpb.31.577
M3 - Article
C2 - 18379044
AN - SCOPUS:41849149702
VL - 31
SP - 577
EP - 582
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 4
ER -