Properties and expression of a multidrug efflux pump AcrAB-KocC from Klebsiella pneumoniae

Dai Wei Li; Motoyasu Onishi; Takanori Kishino; Taira Matsuo; Wakano Ogawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.31.577

Properties and expression of a multidrug efflux pump AcrAB-KocC from Klebsiella pneumoniae

Dai Wei Li, Motoyasu Onishi, Takanori Kishino, Taira Matsuo, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrB_Kp gene and a part of the acrA_Kp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrA_KpB_Kp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrA_Kp B_Kp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAK_KpB_Kp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrA_Kp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.

Original language	English
Pages (from-to)	577-582
Number of pages	6
Journal	Biological and Pharmaceutical Bulletin
Volume	31
Issue number	4
DOIs	https://doi.org/10.1248/bpb.31.577
Publication status	Published - Apr 2008

Keywords

AcrAB-KocC
Drug resistance
Klebsiella pneumoniae
Multidrug efflux pump
Resistance nodulation cell-division

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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10.1248/bpb.31.577

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abstract = "We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrBKp gene and a part of the acrAKp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrAKpBKp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrAKp BKp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAKKpBKp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrAKp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.",

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author = "Li, {Dai Wei} and Motoyasu Onishi and Takanori Kishino and Taira Matsuo and Wakano Ogawa and Teruo Kuroda and Tomofusa Tsuchiya",

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AU - Li, Dai Wei

AU - Onishi, Motoyasu

AU - Kishino, Takanori

AU - Matsuo, Taira

AU - Ogawa, Wakano

AU - Kuroda, Teruo

AU - Tsuchiya, Tomofusa

PY - 2008/4

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AB - We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrBKp gene and a part of the acrAKp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrAKpBKp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrAKp BKp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae, and designated it kocC. It seems that the AcrAKKpBKp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae. We observed a higher level of expression of acrAKp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.

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Properties and expression of a multidrug efflux pump AcrAB-KocC from Klebsiella pneumoniae

Abstract

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