Promotional effects of azathioprine on peripheral B cell lymphomas in BALB/c mice induced by administration of 7,12-dimethylbenz[a]anthracene

Kumi Fujiwara, Tadashi Yoshino, Tadaatsu Akagi, Norihiro Teramoto, Kazuhiko Hayashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


BALB/c mice either were injected subcutaneously with 25 μg/g or 50 μg/g body weight 7,12-dimethylbenz[a]anthracene (DMBA) at the age of 13 weeks, or received repeated s.c. injections of azathioprine (0.1 mg/g) starting from the age of 6 or 18 weeks, or they received treatments with both agents. Of the 317 mice treated, 69 developed lymphomas by the age of 50 weeks. None of the 31 non-treated mice developed lymphomas during this period. The lymphomas, classified according to Pattengale-Taylor's classification for murine lymphomas/leukemias, were examined immunohistochemically and immunogenetically. The lymphomas induced with DMBA showed a male preponderance and a higher incidence of the B cell type, especially in the lymph node and spleen. Azathioprine induced predominantly T-lymphoblastic lymphomas in the thymus and these were found more frequently in females. Treatment with both DMBA and azathioprine remarkably increased the incidence of B lymphomas, especially follicular center cell lymphomas in the spleen, which are the predominant type of spontaneous lymphomas occurring in older BALB/c mice. Some of the treated mice also developed prelymphomatous lesions.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalJournal of cancer research and clinical oncology
Issue number6
Publication statusPublished - Jun 1994


  • 7,12-dimethylbenz[a]anthracene (DMBA)
  • Azathioprine
  • BALB/c mice
  • Immunodeficiency
  • Lymphoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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