Promotion of Ccn2 expression and osteoblastic differentiation by actin polymerization, which is induced by laminar fluid flow stress

Tadashi Honjo, Satoshi Kubota, Hiroshi Kamioka, Yasuyo Sugawara, Yoshihito Ishihara, Takashi Yamashiro, Masaharu Takigawa, Teruko Takano-Yamamoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fluid flow stress (FSS) is a major mechanical stress that induces bone remodeling upon orthodontic tooth movement, whereas CCN family protein 2 (CCN2) is a potent regenerator of bone defects. In this study, we initially evaluated the effect of laminar FSS on Ccn2 expression and investigated its mechanism in osteoblastic MC3T3-E1 cells. The Ccn2 expression was drastically induced by uniform FSS in an intensity dependent manner. Of note, the observed effect was inhibited by a Rho kinase inhibitor Y27632. Moreover, the inhibition of actin polymerization blocked the FSS-induced activation of Ccn2, whereas inducing Factin formation using cytochalasin D and jasplakinolide enhanced Ccn2 expression in the same cells. Finally, Factin formation was found to induce osteoblastic differentiation. In addition, activation of cyclic AMP-dependent kinase, which inhibits Rho signaling, abolished the effect of FSS. Collectively, these findings indicate the critical role of actin polymerization and Rho signaling in CCN2 induction and bone remodeling provoked by FSS.

Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalJournal of Cell Communication and Signaling
Volume6
Issue number4
DOIs
Publication statusPublished - Dec 2012

Fingerprint

jasplakinolide
Bone Remodeling
Plastic flow
Polymerization
CCN Intercellular Signaling Proteins
Actins
Flow of fluids
Tooth Movement Techniques
Cytochalasin D
Adenylate Kinase
rho-Associated Kinases
Mechanical Stress
Cyclic AMP
Bone
Bone and Bones
Chemical activation
Regenerators
Laminar flow
Phosphotransferases
Defects

Keywords

  • Actin polymerization
  • CCN2
  • Connective tissue growth factor
  • CTGF
  • Fluid shear stress
  • Osteoblasts

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Promotion of Ccn2 expression and osteoblastic differentiation by actin polymerization, which is induced by laminar fluid flow stress. / Honjo, Tadashi; Kubota, Satoshi; Kamioka, Hiroshi; Sugawara, Yasuyo; Ishihara, Yoshihito; Yamashiro, Takashi; Takigawa, Masaharu; Takano-Yamamoto, Teruko.

In: Journal of Cell Communication and Signaling, Vol. 6, No. 4, 12.2012, p. 225-232.

Research output: Contribution to journalArticle

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AU - Ishihara, Yoshihito

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AU - Takigawa, Masaharu

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