Promotion of attachment of human bone marrow stromal cells by CCN2

Mitsuaki Ono, Satoshi Kubota, Takuo Fujisawa, Wataru Sonoyama, Harumi Kawaki, Kentaro Akiyama, Masamitsu Oshima, Takashi Nishida, Yasuhiro Yoshida, Kazuomi Suzuki, Masaharu Takigawa, Takuo Kuboki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin αvβ3 antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number1
DOIs
Publication statusPublished - May 25 2007

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Keywords

  • Bone marrow stromal cell
  • CCN2/connective tissue growth factor
  • Cell attachment
  • Hydroxyapatite
  • p38 MAPK

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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