Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma

Hajime Hada, Norio Koide, Takechiyo Morita, Hidenori Shiraha, Toshiyuki Shinji, Masaki Nakamura, Kouzo Ujike, Niro Takayama, Takahiko Oka, Tadashi Hanafusa, Yasuhiro Yumoto, Keisuke Hamazaki, Takao Tsuji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC), an epigenetic change such as hypermethylation of the Rb promoter as reported in retinoblastoma has not been described. Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively. Results: We found no evidence of hypermethylation in the promoter region of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in the patient. Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.

Original languageEnglish
Pages (from-to)1185-1189
Number of pages5
JournalHepato-Gastroenterology
Volume43
Issue number11
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Retinoblastoma Genes
Retinoblastoma
Hepatocellular Carcinoma
Messenger RNA
Genetic Promoter Regions
Proteins
Retinoblastoma Protein
Mutation
Point Mutation
Immunoblotting
Epigenomics
Restriction Fragment Length Polymorphisms
Transcription Factors
Polymerase Chain Reaction

Keywords

  • Hepatocellular carcinoma
  • Hypermethylation
  • Rb gene
  • RFLP

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hada, H., Koide, N., Morita, T., Shiraha, H., Shinji, T., Nakamura, M., ... Tsuji, T. (1996). Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma. Hepato-Gastroenterology, 43(11), 1185-1189.

Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma. / Hada, Hajime; Koide, Norio; Morita, Takechiyo; Shiraha, Hidenori; Shinji, Toshiyuki; Nakamura, Masaki; Ujike, Kouzo; Takayama, Niro; Oka, Takahiko; Hanafusa, Tadashi; Yumoto, Yasuhiro; Hamazaki, Keisuke; Tsuji, Takao.

In: Hepato-Gastroenterology, Vol. 43, No. 11, 1996, p. 1185-1189.

Research output: Contribution to journalArticle

Hada, H, Koide, N, Morita, T, Shiraha, H, Shinji, T, Nakamura, M, Ujike, K, Takayama, N, Oka, T, Hanafusa, T, Yumoto, Y, Hamazaki, K & Tsuji, T 1996, 'Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma', Hepato-Gastroenterology, vol. 43, no. 11, pp. 1185-1189.
Hada, Hajime ; Koide, Norio ; Morita, Takechiyo ; Shiraha, Hidenori ; Shinji, Toshiyuki ; Nakamura, Masaki ; Ujike, Kouzo ; Takayama, Niro ; Oka, Takahiko ; Hanafusa, Tadashi ; Yumoto, Yasuhiro ; Hamazaki, Keisuke ; Tsuji, Takao. / Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma. In: Hepato-Gastroenterology. 1996 ; Vol. 43, No. 11. pp. 1185-1189.
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T1 - Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma

AU - Hada, Hajime

AU - Koide, Norio

AU - Morita, Takechiyo

AU - Shiraha, Hidenori

AU - Shinji, Toshiyuki

AU - Nakamura, Masaki

AU - Ujike, Kouzo

AU - Takayama, Niro

AU - Oka, Takahiko

AU - Hanafusa, Tadashi

AU - Yumoto, Yasuhiro

AU - Hamazaki, Keisuke

AU - Tsuji, Takao

PY - 1996

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