Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma

Hajime Hada, Norio Koide, Takechiyo Morita, Hidenori Shiraha, Toshiyuki Shinji, Masaki Nakamura, Kouzo Ujike, Niro Takayama, Takahiko Oka, Tadashi Hanafusa, Yasuhiro Yumoto, Keisuke Hamazaki, Takao Tsuji

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7 Citations (Scopus)

Abstract

Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC), an epigenetic change such as hypermethylation of the Rb promoter as reported in retinoblastoma has not been described. Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively. Results: We found no evidence of hypermethylation in the promoter region of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in the patient. Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.

Original languageEnglish
Pages (from-to)1185-1189
Number of pages5
JournalHepato-gastroenterology
Volume43
Issue number11
Publication statusPublished - Nov 6 1996
Externally publishedYes

Keywords

  • Hepatocellular carcinoma
  • Hypermethylation
  • RFLP
  • Rb gene

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Hada, H., Koide, N., Morita, T., Shiraha, H., Shinji, T., Nakamura, M., Ujike, K., Takayama, N., Oka, T., Hanafusa, T., Yumoto, Y., Hamazaki, K., & Tsuji, T. (1996). Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma. Hepato-gastroenterology, 43(11), 1185-1189.