Abstract
Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC), an epigenetic change such as hypermethylation of the Rb promoter as reported in retinoblastoma has not been described. Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively. Results: We found no evidence of hypermethylation in the promoter region of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in the patient. Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.
| Original language | English |
|---|---|
| Pages (from-to) | 1185-1189 |
| Number of pages | 5 |
| Journal | Hepato-Gastroenterology |
| Volume | 43 |
| Issue number | 11 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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Keywords
- Hepatocellular carcinoma
- Hypermethylation
- Rb gene
- RFLP
ASJC Scopus subject areas
- Gastroenterology
Cite this
Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma. / Hada, Hajime; Koide, Norio; Morita, Takechiyo; Shiraha, Hidenori; Shinji, Toshiyuki; Nakamura, Masaki; Ujike, Kouzo; Takayama, Niro; Oka, Takahiko; Hanafusa, Tadashi; Yumoto, Yasuhiro; Hamazaki, Keisuke; Tsuji, Takao.
In: Hepato-Gastroenterology, Vol. 43, No. 11, 1996, p. 1185-1189.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma
AU - Hada, Hajime
AU - Koide, Norio
AU - Morita, Takechiyo
AU - Shiraha, Hidenori
AU - Shinji, Toshiyuki
AU - Nakamura, Masaki
AU - Ujike, Kouzo
AU - Takayama, Niro
AU - Oka, Takahiko
AU - Hanafusa, Tadashi
AU - Yumoto, Yasuhiro
AU - Hamazaki, Keisuke
AU - Tsuji, Takao
PY - 1996
Y1 - 1996
N2 - Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC), an epigenetic change such as hypermethylation of the Rb promoter as reported in retinoblastoma has not been described. Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively. Results: We found no evidence of hypermethylation in the promoter region of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in the patient. Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.
AB - Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC), an epigenetic change such as hypermethylation of the Rb promoter as reported in retinoblastoma has not been described. Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively. Results: We found no evidence of hypermethylation in the promoter region of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in the patient. Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.
KW - Hepatocellular carcinoma
KW - Hypermethylation
KW - Rb gene
KW - RFLP
UR - http://www.scopus.com/inward/record.url?scp=10244255087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10244255087&partnerID=8YFLogxK
M3 - Article
C2 - 8908549
AN - SCOPUS:10244255087
VL - 43
SP - 1185
EP - 1189
JO - Acta hepato-splenologica
JF - Acta hepato-splenologica
SN - 0172-6390
IS - 11
ER -