Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae

Yasuhisa Kobayashi; Tomoki Sunobe; Tohru Kobayashi; Yoshitaka Nagahama; Masaru Nakamura

doi:10.1007/s10695-006-0013-6

Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae

Yasuhisa Kobayashi, Tomoki Sunobe, Tohru Kobayashi, Yoshitaka Nagahama, Masaru Nakamura

Faculty of Science

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Despite numerous endocrine studies on sex change in teleost, no general mechanism that mediates sex change has emerged. The gobiid fish, Trimma okinawae, can change sex in both directions repeatedly. This phenomenon of sex change in goby assigns it as an excellent animal model to elucidate the understanding mechanisms of sex change. In hermaphrodite fishes, estrogen plays a particularly important role in natural and experimentally induced sex change. To investigate the role of estrogen in the serial-sex changing fish T. okinawae, we cloned and analyzed the 5′-flanking regions of P450arom genes from goby genome DNA. Both regions have consensus sequences of TATA, CRE and ERE. Ad4 binding site was restricted in the region of P450aromA. These findings indicate that different regulators control the expression of the two P450arom genes.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Fish Physiology and Biochemistry
Volume	31
Issue number	2-3
DOIs	https://doi.org/10.1007/s10695-006-0013-6
Publication status	Published - Apr 2005

Keywords

Gobiid fish
P450 aromatase
Promoter
Sex change
Trimma okinawae

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science

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10.1007/s10695-006-0013-6

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title = "Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae",

abstract = "Despite numerous endocrine studies on sex change in teleost, no general mechanism that mediates sex change has emerged. The gobiid fish, Trimma okinawae, can change sex in both directions repeatedly. This phenomenon of sex change in goby assigns it as an excellent animal model to elucidate the understanding mechanisms of sex change. In hermaphrodite fishes, estrogen plays a particularly important role in natural and experimentally induced sex change. To investigate the role of estrogen in the serial-sex changing fish T. okinawae, we cloned and analyzed the 5′-flanking regions of P450arom genes from goby genome DNA. Both regions have consensus sequences of TATA, CRE and ERE. Ad4 binding site was restricted in the region of P450aromA. These findings indicate that different regulators control the expression of the two P450arom genes.",

keywords = "Gobiid fish, P450 aromatase, Promoter, Sex change, Trimma okinawae",

author = "Yasuhisa Kobayashi and Tomoki Sunobe and Tohru Kobayashi and Yoshitaka Nagahama and Masaru Nakamura",

note = "Funding Information: Acknowledgements This work has been supported by CREST & SORST of JST (Japan Science and Technology Corporation), Bio-Design Program from Ministry of Agriculture, Forestry, and Fisheries, Japan, a Grant-in-Aid for Scientific Research (C16570025) from Ministry of Education, Culture, Sports, Science and Technology, Japan and 21st Century COE program of the University of the Ryukyus. This estrogen-responsive element; CRE, cAMP-responsive element; TATA, box. The position of the gene-specific primers (GSP) are denoted as horizontal arrows and restriction site EcoRV and PvuII is boxed is the contribution from Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus.",

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doi = "10.1007/s10695-006-0013-6",

language = "English",

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T1 - Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae

AU - Kobayashi, Yasuhisa

AU - Sunobe, Tomoki

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AU - Nagahama, Yoshitaka

AU - Nakamura, Masaru

N1 - Funding Information: Acknowledgements This work has been supported by CREST & SORST of JST (Japan Science and Technology Corporation), Bio-Design Program from Ministry of Agriculture, Forestry, and Fisheries, Japan, a Grant-in-Aid for Scientific Research (C16570025) from Ministry of Education, Culture, Sports, Science and Technology, Japan and 21st Century COE program of the University of the Ryukyus. This estrogen-responsive element; CRE, cAMP-responsive element; TATA, box. The position of the gene-specific primers (GSP) are denoted as horizontal arrows and restriction site EcoRV and PvuII is boxed is the contribution from Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus.

PY - 2005/4

Y1 - 2005/4

N2 - Despite numerous endocrine studies on sex change in teleost, no general mechanism that mediates sex change has emerged. The gobiid fish, Trimma okinawae, can change sex in both directions repeatedly. This phenomenon of sex change in goby assigns it as an excellent animal model to elucidate the understanding mechanisms of sex change. In hermaphrodite fishes, estrogen plays a particularly important role in natural and experimentally induced sex change. To investigate the role of estrogen in the serial-sex changing fish T. okinawae, we cloned and analyzed the 5′-flanking regions of P450arom genes from goby genome DNA. Both regions have consensus sequences of TATA, CRE and ERE. Ad4 binding site was restricted in the region of P450aromA. These findings indicate that different regulators control the expression of the two P450arom genes.

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Promoter analysis of two aromatase genes in the serial-sex changing gobiid fish, Trimma okinawae

Abstract

Keywords

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