Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress

Mst Nasrin Akhter Banu, Md Anamul Hoque, Megumi Watanabe-Sugimoto, Ken Matsuoka, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Salt stress causes oxidative damage and cell death in plants. Plants accumulate proline and glycinebetaine (betaine) to mitigate detrimental effects of salt stress. The aim of this study was to investigate the protective effects of proline and betaine on cell death in NaCl-unadapted tobacco (Nicotiana tabacum) Bright Yellow-2 suspension-cultured cells subjected to salt stress. Salt stress increased reactive oxygen species (ROS) accumulation, lipid peroxidation, nuclear deformation and degradation, chromatin condensation, apoptosis-like cell death and ATP contents. Neither proline nor betaine affected apoptosis-like cell death and G1 phase population, and increased ATP contents in the 200 mM NaCl-stressed cells. However, both of them effectively decreased ROS accumulation and lipid peroxidation, and suppressed nuclear deformation and chromatin condensation induced by severe salt stress. Evans Blue staining experiment showed that both proline and betaine significantly suppressed increment of membrane permeability induced by 200 mM NaCl. Furthermore, among the ROS scavenging antioxidant defense genes studied here, mRNA levels of salicylic acid-binding (SAbind) catalase (CAT) and lignin-forming peroxidase (POX) were found to be increased by proline and betaine under salt stress. It is concluded that both proline and betaine provide a protection against NaCl-induced cell death via decreasing level of ROS accumulation and lipid peroxidation as well as improvement of membrane integrity.

Original languageEnglish
Pages (from-to)146-156
Number of pages11
JournalJournal of Plant Physiology
Volume166
Issue number2
DOIs
Publication statusPublished - Jan 30 2009

Fingerprint

betaine
Proline
Tobacco
salt stress
proline
cell death
Cultured Cells
Cell Death
tobacco
Salts
Antioxidants
antioxidant activity
Gene Expression
gene expression
Reactive Oxygen Species
reactive oxygen species
Lipid Peroxidation
cells
lipid peroxidation
Chromatin

Keywords

  • Antioxidant defense gene
  • Cell death
  • Glycinebetaine
  • Proline
  • Salt stress

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress. / Banu, Mst Nasrin Akhter; Hoque, Md Anamul; Watanabe-Sugimoto, Megumi; Matsuoka, Ken; Nakamura, Yoshimasa; Shimoishi, Yasuaki; Murata, Yoshiyuki.

In: Journal of Plant Physiology, Vol. 166, No. 2, 30.01.2009, p. 146-156.

Research output: Contribution to journalArticle

Banu, Mst Nasrin Akhter ; Hoque, Md Anamul ; Watanabe-Sugimoto, Megumi ; Matsuoka, Ken ; Nakamura, Yoshimasa ; Shimoishi, Yasuaki ; Murata, Yoshiyuki. / Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress. In: Journal of Plant Physiology. 2009 ; Vol. 166, No. 2. pp. 146-156.
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