Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells

Md Anamul Hoque, Mst Nasrin Akhter Banu, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Salt stress impairs reactive oxygen species (ROS) and methylglyoxal (MG) detoxification systems, and causes oxidative damage to plants. Up-regulation of the antioxidant and glyoxalase systems provides protection against NaCl-induced oxidative damage in plants. Thiol-disulfide contents, glutathione content and its associated enzyme activities involved in the antioxidant defense and glyoxalase systems, and protein carbonylation in tobacco Bright Yellow-2 cells grown in suspension culture were investigated to assess the protection offered by proline and glycinebetaine against salt stress. Salt stress increased protein carbonylation, contents of thiol, disulfide, reduced (GSH) and oxidized (GSSG) forms of glutathione, and the activity of glutathione-S-transferase and glyoxalase II enzymes, but decreased redox state of both thiol-disulfide and glutathione, and the activity of glutathione peroxidase and glyoxalase I enzymes involved in the ROS and MG detoxification systems. Exogenous application of proline or glycinebetaine resulted in a reduction of protein carbonylation, and in an increase in glutathione redox state and activity of glutathione peroxidase, glutathione-S-transferase and glyoxalase I under salt stress. Neither proline nor glycinebetaine, however, had any direct protective effect on NaCl-induced GSH-associated enzyme activities. The present study, therefore, suggests that both proline and glycinebetaine provide a protective action against NaCl-induced oxidative damage by reducing protein carbonylation, and enhancing antioxidant defense and MG detoxification systems.

Original languageEnglish
Pages (from-to)813-824
Number of pages12
JournalJournal of Plant Physiology
Volume165
Issue number8
DOIs
Publication statusPublished - May 26 2008

Fingerprint

Protein Carbonylation
Pyruvaldehyde
betaine
Proline
Tobacco
salt stress
Glutathione Disulfide
proline
glutathione
Cultured Cells
tobacco
Salts
Antioxidants
antioxidant activity
thiols
Lactoylglutathione Lyase
sulfides
Sulfhydryl Compounds
Enzymes
Glutathione Peroxidase

Keywords

  • Antioxidant defense
  • Glycinebetaine
  • Glyoxalase
  • Proline
  • Salt stress

ASJC Scopus subject areas

  • Plant Science

Cite this

Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells. / Hoque, Md Anamul; Banu, Mst Nasrin Akhter; Nakamura, Yoshimasa; Shimoishi, Yasuaki; Murata, Yoshiyuki.

In: Journal of Plant Physiology, Vol. 165, No. 8, 26.05.2008, p. 813-824.

Research output: Contribution to journalArticle

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